Shelf-Life of e-Lysyl-3-(Trimethylstannyl)Benzamide Immunoconjugates, Precursors for At-211 Labeling of Antibodies

Artikel i vetenskaplig tidskrift, refereegranskad
Författare
Emma Aneheim | Institutionen för kliniska vetenskaper, Avdelningen för radiofysik
Jenny Halleröd | Institutionen för kliniska vetenskaper, Avdelningen för radiofysik
Per Albertsson | Institutionen för kliniska vetenskaper, Avdelningen för onkologi
H. Jensen | Extern
Stellan Holgersson | Extern
Sture Lindegren | Institutionen för kliniska vetenskaper, Avdelningen för radiofysik
Publikationsår
2015
Publicerad i
Cancer Biotherapy and Radiopharmaceuticals, 30 (1), 41-45
Sammanfattning
Astatine-211 is possibly the most promising radionuclide for targeted alpha-particle therapy when it comes to the treatment of occult disseminated cancer. Preclinical research has proven effective, and patient studies have been initiated based on these results. However, a lack of production capacity and the complex radiochemistry of At-211 are major obstacles for research and prospective clinical applications. In the present study, astatination of immunoconjugates, already prepared well in advance before radiolabeling, was performed to investigate the possibility of formulating a kit-like reagent for the production of At-211 radiopharmaceuticals. The shelf-life of e-lysyl-3-(trimethylstannyl)benzamide immunoconjugates was evaluated, that is, the effect of different storage times on the quality of the immunoconjugates. The quality being referred to is the capacity to maintain a good radiochemical yield and good cell-binding property after labeling with At-211. The stability of the conjugates was found to be pH dependent with high stability at pH >= 7 and less stability at pH <= 5.5. The immunoconjugates (based on trastuzumab) could be kept for more than 3 months in a phosphate buffered saline solution (pH 7.4) at 4 degrees C before labeling, without compromising the quality of the labeled product. The conjugates are also unaffected by storage at -20 degrees C. Conjugates with a good shelf-life compatible with distant shipping as well as improved radiochemistry are important steps to facilitate further clinical progress with At-211.
ISSN
1084-9785
Ämnesord
  • MEDICIN OCH HÄLSOVETENSKAP | Klinisk medicin | Radiologi och bildbehandling
  • MEDICIN OCH HÄLSOVETENSKAP | Medicinska grundvetenskaper | Farmakologi och toxikologi
Nyckelord
immunoconjugate, labeling, antibodies, shelf-life, astatine-211
Språk
Engelska
Publikations-ID
213443
Identifikatorer