transparent gif


Ej inloggad.

Göteborgs universitets publikationer

Embryonic stem cells express growth hormone receptors: regulation by retinoic acid.

Författare och institution:
Claes Ohlsson (Institutionen för invärtesmedicin); Karina Lövstedt (Institutionen för laboratoriemedicin); P V Holmes (-); Anders Nilsson (Institutionen för de kirurgiska disciplinerna, Avdelningen för ortopedi); Lena M S Carlsson (Institutionen för invärtesmedicin); Jan Törnell (Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi)
Publicerad i:
Endocrinology, 133 ( 6 ) s. 2897-903
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Embryonic and fetal growth are generally considered to be independent of pituitary GH. However, it has been demonstrated recently that 18-day-old rat embryos and rat fetuses express GH receptors, suggesting that GH could play a role in early development. The aim of the present investigation was to determine whether preimplantation embryos also express GH receptors. Germ line competent mouse embryonic stem (ES) cells and cultured mouse preimplantation embryos were examined with Northern blot analysis, RNAse-protection solution-hybridization assays, reverse transcription/polymerase chain reaction assays and immunohistochemistry for the detection of GH receptors. Northern blot analysis of ES cells using a probe corresponding to the extracellular domain of the GH receptor demonstrated the presence of two transcripts (1.2 and 4.5 kilobases). The RNAse-protection solution-hybridization assay revealed that ES cells express approximately one sixth of the GH-receptor messenger RNA (mRNA) levels expressed in liver from pregnant mice. Treatment of cultured ES cells with retinoic acid (100 nM) for 6 days increased GH-receptor mRNA levels (P < 0.01). GH-receptor mRNA was further identified in ES cells, preimplantation embryos, muscle, liver, and placenta by a reverse transcription/polymerase chain reaction assay. In humans it has previously been shown that exon 3 of the GH-receptor is deleted in the placenta. However, none of the studied mouse tissues had a deletion of the GH-receptor mRNA corresponding to exon 3 of the human GH receptor. GH-receptor immunoreactivity was identified on the cultured ES-cells by immunohistochemistry. In conclusion, we have in the present study shown that germline competent ES cells and preimplantation mouse embryos express the GH receptor transcript and that this transcription is increased by retinoic acid in ES cells. Furthermore, the presence of GH-receptor immunoreactivity on the ES cells indicates that the GH-receptor transcript is translated.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Animals, Base Sequence, Blotting, Northern, Embryo, Mammalian, cytology, metabolism, Humans, Immunohistochemistry, Mice, Molecular Sequence Data, Nucleic Acid Hybridization, Polymerase Chain Reaction, RNA, Messenger, metabolism, Receptors, Somatotropin, genetics, metabolism, Ribonucleases, Sequence Homology, Nucleic Acid, Stem Cells, metabolism, Tissue Distribution, Transcription, Genetic, Tretinoin, pharmacology
Postens nummer:
Posten skapad:
2009-03-19 09:28
Posten ändrad:
2011-01-20 09:58

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007