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Göteborgs universitets publikationer

Characterization of 4-hydroxyphenylpyruvate dioxygenase. Primary structure of the Pseudomonas enzyme.

Författare och institution:
Ulla Rüetschi (Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin); Birgit Odelhög (Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin); Sven Lindstedt (Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin); Jane Barros-Söderling (-); B Persson (-); Hans Jörnvall (-)
Publicerad i:
European journal of biochemistry / FEBS, 205 ( 2 ) s. 459-66
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The primary structure of Pseudomonas 4-hydroxyphenylpyruvate dioxygenase was determined. Sequence degradation of the intact protein and of peptides from three different digests of the carboxymethylated protein established a 357-residue polypeptide chain with a free alpha-amino group. Hydroxylamine cleavage at a single Asn-Gly sequence was useful. Comparisons with known structures in data banks revealed no close relationship with other characterized proteins. The human enzyme has a related composition, suggesting that also the eukaryotic form belongs to this protein type, but with a blocked N-terminus like in many other eukaryotic intracellular proteins. Secondary structure predictions suggest an alpha/beta mixed structure, fairly typical of globular proteins, without long segments of hydrophobicity or charge, although a region in the middle of the C-terminal third of the subunit appears to have the most extreme properties. A ferric centre, correlating with enzyme activity and absorbance at 595 nm, has previously been assigned to tyrosinate coordination. The Tyr and His distributions, and the position of a single Cys residue, all suggest a few likely sites, outside the C-terminal segment, for this centre.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinsk bioteknologi ->
Medicinsk bioteknologi (med inriktning mot cellbiologi)
4-Hydroxyphenylpyruvate Dioxygenase, chemistry, Amino Acid Sequence, Chromatography, Gel, Chromatography, High Pressure Liquid, Cyanogen Bromide, Humans, Hydroxylamine, Hydroxylamines, Molecular Sequence Data, Peptide Fragments, isolation & purification, Protein Conformation, Pseudomonas, enzymology, Trypsin
Postens nummer:
Posten skapad:
2009-03-11 14:05
Posten ändrad:
2011-01-20 09:59

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