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Göteborgs universitets publikationer

Methodological issues on the use of urinary alpha-1-microglobuline in epidemiological studies.

Författare och institution:
Lena Andersson (Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa); Börje Haraldsson (Institutionen för medicin, avdelningen för molekylär och klinisk medicin); Caroline Johansson (Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa); Lars Barregård (Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa)
Publicerad i:
Nephrology Dialysis Transplantation, 23 ( 4 ) s. 1252-1256
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Background. Alpha-1-microglobulin (A1M) is a low molecular weight protein that can be measured in urine and used as a marker for tubular function, assuming that the normal variability within and between individuals is known. The aims of this study were to investigate this variability, to find the optimal way of sampling and quantifying A1M in spot urine samples to reflect the 24 h excretion and to examine storage stability. Method. Timed urine specimens were collected from 29 healthy volunteers at fixed time points over 24 h on two separate days. Volumes, creatinine and specific gravity were determined. All samples were analysed with a commercial ELISA for A1M. Results. We found a clear diurnal variation in A1M excretion rate and a gender effect (higher in males). The excretion rate was higher in the daytime, with high urinary flow, compared to overnight values. A1M excretion in spot urine samples was highly correlated with the 24 h excretion at all times except 22:00 in male subjects. Urinary A1M adjusted for creatinine concentration correlated well with the 24 h excretion. Variability within individuals was only 20% of the total variability in 24 h A1M excretion, but 43% in first morning urine. Expressed as CV, the intra-individual variability (between days) was 29% in 24 h excretion. Conclusion. We conclude that diurnal variation and gender should be taken into account when comparing groups. Moreover, in spot samples (e.g. first morning samples) adjustment of A1M for creatinine or specific gravity is a reliable alternative to 24 h urine.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin ->
Urologi och njurmedicin ->
Hälsovetenskaper ->
Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi ->
Folkhälsomedicinska forskningsområden
Hälsovetenskaper ->
Miljömedicin och yrkesmedicin ->
Alpha-1-microglobulin; diurnal; protein HC; urine sampling; variability; Adult, Alpha-Globulins/*urine, Biological Markers/*urine, Circadian Rhythm/*physiology, Creatinine/urine, Enzyme-Linked Immunosorbent Assay, Female, Follow-Up Studies, Humans, Kidney Tubules/*metabolism, Male, Middle Aged, Morbidity/trends, Observer Variation, Prognosis, Reference Values, Renal Insufficiency/*epidemiology/*urine, Reproducibility of Results, Sweden/epidemiology
Postens nummer:
Posten skapad:
2009-01-14 08:11
Posten ändrad:
2011-01-20 09:58

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