transparent gif


Ej inloggad.

Göteborgs universitets publikationer

Interleukin-17A mediates acquired immunity to pneumococcal colonization.

Författare och institution:
Ying-Jie Lu (-); Jane Gross (-); Debby Bogaert (-); Adam Finn (-); Linda Bagrade (-); Qibo Zhang (-); Jay K Kolls (-); Amit Srivastava (-); Anna Lundgren (Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi); Sophie Forte (-); Claudette M Thompson (-); Kathleen F Harney (-); Porter W Anderson (-); Marc Lipsitch (-); Richard Malley (-)
Publicerad i:
PLoS pathogens, 4 ( 9 ) s. e1000159
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Although anticapsular antibodies confer serotype-specific immunity to pneumococci, children increase their ability to clear colonization before these antibodies appear, suggesting involvement of other mechanisms. We previously reported that intranasal immunization of mice with pneumococci confers CD4+ T cell-dependent, antibody- and serotype-independent protection against colonization. Here we show that this immunity, rather than preventing initiation of carriage, accelerates clearance over several days, accompanied by neutrophilic infiltration of the nasopharyngeal mucosa. Adoptive transfer of immune CD4+ T cells was sufficient to confer immunity to naïve RAG1(-/-) mice. A critical role of interleukin (IL)-17A was demonstrated: mice lacking interferon-gamma or IL-4 were protected, but not mice lacking IL-17A receptor or mice with neutrophil depletion. In vitro expression of IL-17A in response to pneumococci was assayed: lymphoid tissue from vaccinated mice expressed significantly more IL-17A than controls, and IL-17A expression from peripheral blood samples from immunized mice predicted protection in vivo. IL-17A was elicited by pneumococcal stimulation of tonsillar cells of children or adult blood but not cord blood. IL-17A increased pneumococcal killing by human neutrophils both in the absence and in the presence of antibodies and complement. We conclude that IL-17A mediates pneumococcal immunity in mice and probably in humans; its elicitation in vitro could help in the development of candidate pneumococcal vaccines.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper
Klinisk medicin ->
Adoptive Transfer, Adult, Animals, CD4-Positive T-Lymphocytes, immunology, transplantation, Child, Female, Humans, Immunity, immunology, Interleukin-17, immunology, Mice, Mice, Inbred C57BL, Neutrophils, immunology, Pneumococcal Infections, immunology, Streptococcus pneumoniae, immunology
Postens nummer:
Posten skapad:
2009-01-12 17:33
Posten ändrad:
2013-01-14 18:28

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007