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Göteborgs universitets publikationer

Cytolethal distending toxin upregulates RANKL expression in Jurkat T-cells.

Författare och institution:
Georgios N Belibasakis (-); Monica Brage (-); Teresa Lagergård (Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi); Anders Johansson (-)
Publicerad i:
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 116 ( 6 ) s. 499-506
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Cytolethal distending toxin, a bacterial exotoxin produced by a number of Gram-negative species, causes growth arrest and morphological alterations in host cells. Among these species are Haemophilus ducreyi, the etiological agent of chancroid, and the periodontal pathogen Aggregatibacter actinomycetemcomitans, highly implicated in localized aggressive periodontitis. CDT induces receptor activator of NF-kappaB ligand (RANKL) expression in periodontal fibroblasts, the key bone-resorbing cytokine. T-cells are actively involved in localized inflammation-induced bone destruction, including periodontitis. The aim of this study was to investigate the effects of purified CDT on the expression of RANKL and its decoy receptor osteoprotegerin (OPG), in the Jurkat T-cell line. Quantitative real-time PCR indicated that 100 pg/ml of purified H. ducreyi CDT upregulated RANKL mRNA expression by 2.2-fold, after 24 h of exposure. This increase was corroborated by a 2.0-fold increase in RANKL protein release, as determined by ELISA. OPG was not detected in this experimental system. In conclusion, CDT enhances RANKL expression in T-cells, denoting that these cells are a potential target for the toxin and strengthening the potential link between this virulence factor and mechanisms associated with localized bone resorption.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Mikrobiologi inom det medicinska området
Bacterial Toxins, isolation & purification, metabolism, toxicity, Cell Cycle, Cell Line, Tumor, Haemophilus ducreyi, Humans, Jurkat Cells, NF-kappa B, metabolism, Osteoprotegerin, metabolism, RANK Ligand, metabolism, T-Lymphocytes, metabolism
Postens nummer:
Posten skapad:
2009-01-07 14:53
Posten ändrad:
2012-06-26 13:39

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