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Göteborgs universitets publikationer

Persistent FoxE3 expression blocks cytoskeletal remodeling and organelle degradation during lens fiber differentiation.

Författare och institution:
Henrik Landgren (Institutionen för cell- och molekylärbiologi); Åsa Blixt (Institutionen för cell- och molekylärbiologi); Peter Carlsson (Institutionen för cell- och molekylärbiologi)
Publicerad i:
Investigative ophthalmology & visual science, 49 ( 10 ) s. 4269-77
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
PURPOSE: The anterior hemisphere of the lens is covered by an epithelial monolayer that acts as the stem cell population for lens fiber progenitors. Foxe3, a forkhead transcription factor, is essential for proliferation and survival of the epithelial cells, and cessation of Foxe3 expression at the lens equator coincides with the cell cycle arrest that marks initiation of fiber differentiation. In this study, the consequences of persistent Foxe3 expression during fiber differentiation was investigated. METHODS: The alpha-A-crystallin (Cryaa) promoter was used to drive transgenic expression of Foxe3 in murine differentiating lens fibers. RESULTS: Transgenic mice have a dramatically disturbed lens histology and grave cataracts. Microarray transcript profiling showed an increase of mRNAs normally enriched in epithelial cells, consistent with an epithelialization of the transgenic fibers. Some aspects of fiber differentiation were unaffected, such as the expression of alpha- and beta-crystallins and aquaporins, whereas cytoskeletal remodeling, cell adhesion, organelle degradation, and antimitotic signaling were compromised. CONCLUSIONS: Proper inactivation of FoxE3 expression at the lens equator is important for many aspects of fiber differentiation, and persistent expression leads to a partial epithelialization of fiber cells, with severe consequences for lens function.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Biologiska vetenskaper ->
Biokemi och molekylärbiologi ->
Cell- och molekylärbiologi ->
Animals, Blotting, Southern, Cell Adhesion, physiology, Cell Differentiation, physiology, Cytoskeleton, physiology, Forkhead Transcription Factors, genetics, metabolism, Gene Expression Profiling, Gene Expression Regulation, physiology, In Situ Hybridization, Lens, Crystalline, cytology, metabolism, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Oligonucleotide Array Sequence Analysis, Organelles, metabolism, Phenotype, Polymerase Chain Reaction, RNA, Messenger, metabolism
Postens nummer:
Posten skapad:
2008-12-18 13:14
Posten ändrad:
2010-01-26 10:52

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