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Göteborgs universitets publikationer

Prolonged eosinophil production after allergen exposure in IFN-gammaR KO mice is IL-5 dependent.

Författare och institution:
Lin-Ling Zhao (Institutionen för medicin, avdelningen för invärtesmedicin); Jan Lötvall (Institutionen för medicin, avdelningen för invärtesmedicin); Anders Lindén (Institutionen för medicin, avdelningen för invärtesmedicin); Masafumi Tomaki (Institutionen för medicin, avdelningen för invärtesmedicin); Margareta Sjöstrand (Institutionen för medicin, avdelningen för invärtesmedicin); Apostolos Bossios (Institutionen för medicin, avdelningen för invärtesmedicin)
Publicerad i:
Scandinavian journal of immunology, 67 ( 5 ) s. 480-8
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Asthma is a T helper 2 (Th2)-driven inflammatory process characterized by eosinophilia. Prolonged airway eosinophilia is commonly observed in asthma exacerbations. Our aim was to evaluate whether eosinophilia in prolonged allergic inflammation is associated with a continuous supply of new eosinophils to the airways, and how this is regulated. Ovalbumin (OVA)-sensitized interferon-gamma receptor knockout mice (IFN-gammaR KO), known to maintain a long-lasting eosinophilia after allergen exposure, were compared to wild type (wt) controls. Animals were exposed to OVA or phosphate-buffered saline on three consecutive days, and bone marrow (BM), blood and bronchoalveolar lavage (BAL) samples were collected 24 h, 7 and 21 days later. Newly produced cells were labelled using bromodeoxyuridine (BrdU). Serum IL-5 was measured and its role was investigated by administration of a neutralizing anti-IL-5 antibody. In-vitro eosinophilopoiesis was examined in both groups by a colony-forming assay. Allergen challenge increased eosinophils in BM, blood and BAL, in both IFN-gammaR KO and wt mice, both 24 h and 7 days after the last allergen exposure. At 21 days after the last exposure, only IFN-gammaR KO mice maintained significantly increased eosinophil numbers. Approximately 50% of BAL granulocytes in IFN-gammaR KO were produced during the last 6 days. Interleukin (IL)-5 concentration was increased in IFN-gammaR KO mice, and anti-IL-5 reduced eosinophil numbers in all compartments. Increased numbers of eosinophil colonies were observed in IFN-gammaR KO mice after allergen exposure versus controls. In this model of a Th2-driven prolonged allergic eosinophilia, new eosinophils contribute to the extended inflammation in the airways by enhanced BM eosinophilopoiesis in an IL-5-dependent manner.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin ->
Lungmedicin och allergi
Postens nummer:
Posten skapad:
2008-04-16 09:06
Posten ändrad:
2011-01-20 09:59

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