transparent gif


Ej inloggad.

Göteborgs universitets publikationer

Human small intestinal epithelial cells constitutively express the key elements for antigen processing and the production of exosomes.

Författare och institution:
Xiao Ping Lin (-); Nina Almqvist (Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning); Esbjörn Telemo (Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning)
Publicerad i:
Blood cells, molecules & diseases, 35 ( 2 ) s. 122-8
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
In humans, the small intestinal epithelial cells (IEC) have a high constitutive expression of MHC class II (MHC II), and contains lysosomes. The IEC also contains MHC II rich multivesicular compartments and has been shown to produce exosomes. This suggests a role for the IEC in antigen processing and presentation either directly or indirectly by the production of exosomes. However, the presence and localisation in the IEC of other key molecules involved in this process has not been studied previously. In the present work, we have investigated small intestinal biopsies from healthy adults and the HT29 IEC cell line with monoclonal antibodies against molecules involved in the antigen processing/presenting systems and molecules typically found on exosomes derived from professional APCs and IECs. Immunohistology was performed to study the expression and localisation of MHC II (HLA-DR), HLA-DM, MHC I (HLA-ABC), CD1d, Invariant chain, Lamp-1, CD68, CD63, B7.1, B7.2, ICAM-1, Cathepsin D/S/L and the IEC specific marker A33 in the IECs. We found that the IECs from the biopsies constitutively express MHC II, HLA-DM, MHC I, Invariant chain, Lamp-1, CD 68, CD63 and A33, and these markers were also found in the IFN-g treated HT-29 cells. All these molecules were found apically in the IECs of the biopsies, localised mainly in vesicular structures. Interestingly, in the baso-latereral area of the IEC, only MHC II, MHC I, Lamp 1, CD68, CD63 and A33 were found and also here with vesicular staining pattern which matches the molecules previously found on exosomes derived professional APCs and human IEC lines. CD1d, B7, ICAM-1, CD9 and cathepsin D and L were absent in the IEC compartment, but cathepsin S showed a relatively weak staining in the apical part of the IEC. The staining pattern and the morphological localisation of these markers suggest a prominent antigen processing/loading and trafficking compartment, and a possible baso-lateral release of exosomes in the normal human IEC.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Immunologi inom det medicinska området
Adult, Aged, Antigen Presentation, Antigens, Surface, analysis, Cell Compartmentation, Endosomes, immunology, metabolism, secretion, Epithelial Cells, immunology, Exocytosis, Female, Humans, Immunohistochemistry, Immunophenotyping, Intestinal Mucosa, cytology, immunology, Intestine, Small, cytology, Male, Middle Aged
Postens nummer:
Posten skapad:
2008-01-17 10:19
Posten ändrad:
2011-01-20 09:58

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007