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Göteborgs universitets publikationer

Circulating eosinophils in asthma, allergic rhinitis, and atopic dermatitis lack morphological signs of degranulation

Författare och institution:
M. Malm-Erjefält (-); L. Greiff (-); J. Ankerst (-); M. Andersson (-); J. Wallengren (-); L. O. Cardell (-); Sabina Rak (Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi); C. G. Persson (-); J. S. Erjefält (-)
Publicerad i:
Clin Exp Allergy, 35 ( 10 ) s. 1334-40.
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Summary Background In allergic diseases, eosinophils in affected tissues release granule proteins with cytotoxic, immunoregulatory, and remodelling-promoting properties. From recent observations, it may be assumed that eosinophils degranulate already in circulating blood. If degranulation occurs in the circulation, this could contribute to widespread systemic effects and provide an important marker of disease. Objective To determine the degranulation status of circulating eosinophils in common allergic diseases. Methods Using a novel approach of whole blood fixation and leucocyte preparation, the granule morphology of blood eosinophils from healthy subjects, non-symptomatic patients, symptomatic patients with asthma, asthma and Churg-Strauss syndrome, allergic rhinitis, and atopic dermatitis was evaluated by transmission electron microscopy (TEM) and eosinophil peroxidase (TEM) histochemistry. Plasma and serum levels of eosinophil cationic protein were measured by fluoroenzymeimmunoassay. Selected tissue biopsies were examined by TEM. Results Regardless of symptoms, circulating eosinophils from allergic patients showed the same granule morphology as cells from healthy subjects. The majority of eosinophil-specific granules had preserved intact electron-density (96%; range: 89-98%), while the remaining granules typically exhibited marginal coarsening or mild lucency of the matrix structure. Abnormalities of the crystalline granule core were rarely detected. Furthermore, granule matrix alterations were not associated with any re-localization of intracellular EPO or increase in plasma eosinophil cationic protein. By contrast, eosinophils in diseased tissues exhibited cytolysis (granule release through membrane rupture) and piecemeal degranulation (loss of granule matrix and core structures). Conclusion In symptomatic eosinophilic diseases, circulating blood eosinophils retain their granule contents until they have reached their target organ.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin ->
Lungmedicin och allergi
Postens nummer:
Posten skapad:
2007-11-20 13:20
Posten ändrad:
2010-01-26 11:04

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