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Göteborgs universitets publikationer

Microarray analysis of blood microvessels from PDGF-B and PDGF-Rbeta mutant mice identifies novel markers for brain pericytes.

Författare och institution:
Cecilia Bondjers (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Liqun He (-); Minoru Takemoto (-); Jenny Norlin (-); Noomi Asker (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Mats Hellström (-); Per Lindahl (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi & Wallenberglaboratoriet & Institutionen för medicin, avdelningen för molekylär och klinisk medicin); Christer Betsholtz (-)
Publicerad i:
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20 ( 10 ) s. 1703-5
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Normal blood microvessels are lined by pericytes, which contribute to microvessel development and stability through mechanisms that are poorly understood. Pericyte deficiency has been implicated in the pathogenesis of microvascular abnormalities associated with diabetes and tumors. However, the unambiguous identification of pericytes is still a problem because of cellular heterogeneity and few available molecular markers. Here we describe an approach to identify pericyte markers based on transcription profiling of pericyte-deficient brain microvessels isolated from platelet-derived growth factor (PDGF-B)-/- and PDGF beta receptor (PDGFRbeta)-/- mouse mutants. The approach was validated by the identification of known pericyte markers among the most down-regulated genes in PDGF-B-/- and PDGFRbeta-/- microvessels. Of candidates for novel pericyte markers, we selected ATP-sensitive potassium-channel Kir6.1 (also known as Kcnj8) and sulfonylurea receptor 2, (SUR2, also known as Abcc9), both part of the same channel complex, as well as delta homologue 1 (DLK1) for in situ hybridization, which demonstrated their specific expression in brain pericytes of mouse embryos. We also show that Kir6.1 is highly expressed in pericytes in brain but undetectable in pericytes in skin and heart. The three new brain pericyte markers are signaling molecules implicated in ion transport and intercellular signaling, potentially opening new windows on pericyte function in brain microvessels.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
ATP-Binding Cassette Transporters, analysis, Animals, Biological Markers, analysis, Brain, blood supply, cytology, Capillaries, cytology, Embryo, Gene Expression Profiling, Intercellular Signaling Peptides and Proteins, Membrane Proteins, analysis, Mice, Mice, Mutant Strains, Microarray Analysis, methods, Pericytes, chemistry, Potassium Channels, Inwardly Rectifying, analysis, Proto-Oncogene Proteins c-sis, deficiency, Receptor, Platelet-Derived Growth Factor beta, deficiency, Repressor Proteins, analysis, Tissue Distribution
Postens nummer:
Posten skapad:
2007-11-12 11:47
Posten ändrad:
2011-01-20 09:59

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