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Göteborgs universitets publikationer

Voluntary physical exercise and coronary flow velocity reserve: a transthoracic colour Doppler echocardiography study in spontaneously hypertensive rats

Författare och institution:
Ulrika Hägg Samuelsson (Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi); Julia Grönros (Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi); Johannes Wikström (Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi); Ingibjörg H Jonsdottir (Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi); Göran Bergström (Hjärt-kärlinstitutionen); Li-Ming Gan (Hjärt-kärlinstitutionen)
Publicerad i:
Clin Sci (Lond), 109 ( 3 ) s. 325-34
0143-5221 (Print)
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
In the present study, we have developed and demonstrated a coronary artery imaging protocol in rats using transthoracic high-frequency CDE (colour Doppler echocardiography) to investigate the potential direct effects of exercise on CFVR (coronary flow velocity reserve). SHR (spontaneously hypertensive rats) performed voluntary exercise for 6 weeks. Rats were then submitted to ultrasonographic examination and CFVR measurements. The LAD (left anterior descending coronary artery) was visualized using transthoracic CDE in a modified parasternal long-axis view. Doppler measurement was made in mid-LAD during baseline and adenosine-induced hyperaemic condition. Gene and protein expression in cardiac tissue were studied using real-time PCR and immunohistochemistry. Adenosine infusion significantly (P<0.001, as determined by ANOVA) decreased HR, without affecting blood pressure in anaesthetized SHR. A significantly greater adenosine dose-dependent response was seen in exercised rats compared with controls (P=0.02, as determined by ANOVA). The baseline flow velocity in mid-LAD was 0.33+/-0.06 and 0.41+/-0.14 m/s in the exercised and control animals respectively (P value was not significant). The maximum adenosine-induced response was reached at a dose of 140 of body weight.min-1, and CFVR averaged at 2.6+/-0.53 and 1.5+/-0.24 in exercised and control animals respectively (P<0.01). Gene expression of CuZnSOD was up-regulated by 21% in exercised animals compared with controls (1.1+/-0.16 compared with 0.89+/-0.09; P<0.01), whereas eNOS expression was unchanged. In conclusion, CFVR in rats can be non-invasively assessed using CDE with high feasibility. Physical exercise is associated with improved CFVR and antioxidative capacity in SHR.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Animals, Blood Flow Velocity, Blood Pressure, Coronary Vessels/*physiopathology/ultrasonography, Disease Models, Animal, Echocardiography, Doppler, Color, Feasibility Studies, Gene Expression Regulation, Enzymologic, Heart Rate, Hypertension/enzymology/*physiopathology/ultrasonography, Male, Motor Activity/*physiology, Rats, Rats, Inbred SHR, Rats, Inbred WKY, Running, Superoxide Dismutase/genetics/metabolism
Postens nummer:
Posten skapad:
2007-10-30 14:21
Posten ändrad:
2011-01-20 09:59

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