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Göteborgs universitets publikationer

Peritoneal dialysis fluid-induced angiogenesis in rat mesentery is increased by lactate in the presence or absence of glucose.

Författare och institution:
Ann Albrektsson (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Farhan Bazargani (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Anders Wieslander (-); Magnus Braide (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi)
Publicerad i:
ASAIO journal (American Society for Artificial Internal Organs : 1992), 52 ( 3 ) s. 276-81
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Angiogenesis may be an important mechanism behind the functional deterioration of the peritoneum leading to ultrafiltration failure in peritoneal dialysis. The present study was designed to compare the angiogenic properties of lactate-, bicarbonate-, and pyruvate-buffered fluids, evaluated separately with and without glucose. Five different fluids (lactate and bicarbonate with and without 2.5% glucose and pyruvate without glucose) were studied for 5 weeks of twice-daily injections in rats. The respective buffers (40 mmol/l) were adjusted to pH 7.2, and sodium, chloride, calcium, and magnesium were present at standard concentrations. The mesenteric window model, based on observation of the translucent peritoneal sections of the small intestine mesentery, was used for immunohistochemical imaging of microvessels (RECA-1 antigen) and macrophages (ED1 and ED2 antigens). All fluids induced angiogenesis as compared with untreated controls. The lactate-buffered fluids induced larger vascularized zones than did their bicarbonate- and pyruvate-buffered counterparts. Angiogenesis was accompanied by a local recruitment of ED1 macrophages from blood. Addition of glucose to the lactate- and bicarbonate-buffered fluids did not seem to alter their pro-angiogenic properties. In conclusion, intraperitoneal exposure to lactate buffer, compared with bicarbonate, stimulates angiogenesis in the presence or absence of glucose.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Animals, Bicarbonates, pharmacology, Buffers, Dialysis Solutions, pharmacology, Glucose, pharmacology, Hydrogen-Ion Concentration, Immunohistochemistry, Lactates, pharmacology, Macrophages, Peritoneal, drug effects, Male, Mesentery, blood supply, Microscopy, Fluorescence, Neovascularization, Physiologic, drug effects, Peritoneal Dialysis, methods, Pyruvic Acid, pharmacology, Rats, Rats, Sprague-Dawley, Time Factors
Postens nummer:
Posten skapad:
2007-10-30 11:58

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