transparent gif


Ej inloggad.

Göteborgs universitets publikationer

Localization of lipids in the aortic wall with imaging TOF-SIMS

Författare och institution:
Per Malmberg (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); K. Borner (-); Yun Chen (Institutionen för medicin, avdelningen för molekylär och klinisk medicin); Peter Friberg (Institutionen för medicin, avdelningen för molekylär och klinisk medicin); B. Hagenhoff (-); Jan-Eric Månsson (-); Håkan Nygren (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi)
Publicerad i:
Biochim Biophys Acta, 1771 ( 2 ) s. 185-95
0006-3002 (Print)
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) was utilized to address the issue of localization of lipids and inorganic ions in healthy rat aorta and human atherosclerotic plaque. Pieces of rat aorta were high pressure frozen, freeze-fractured and freeze dried. The samples were analyzed by imaging TOF-SIMS equipped with a Bi(1-7)(+)-source. Reference lipid samples were analyzed and compared to data obtained by analysis of the rat aorta samples. Fatty acids, cholesterol, oxysterol and diacylglycerols were detected and localized. A heterogeneous lipid distribution could be shown in the aorta, where the lamellae of the aorta, distinguished by imaging of CN(-), appeared enriched in cholesterol, oxysterol and diacylglycerols, while the smooth muscle tissue, identified by imaging of PO(3), appeared enriched in phosphocholine. Palmitic/palmitoleic acid and stearic/oleic acid appeared to be heterogeneously distributed over the aorta with high concentration areas located especially in the tunica media region of the aorta. Human atherosclerotic plaque showed an irregular cholesterol distribution mainly located in spots in the intima region with elongated diacylglycerol regions located mainly in the media region.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Animals, Aorta/*metabolism/ultrastructure, Atherosclerosis/metabolism, Cholesterol/metabolism, Fatty Acids, Monounsaturated/metabolism, Freeze Drying, Humans, Lipids/*analysis, Oleic Acid/metabolism, Palmitic Acid/metabolism, Rats, Rats, Sprague-Dawley, Spectrometry, Mass, Secondary Ion/*methods, Stearic Acids/metabolism
Postens nummer:
Posten skapad:
2007-10-29 14:27
Posten ändrad:
2015-06-12 13:21

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007