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Sentinel-base DNA genotyping using multiple sequencing primers for high-risk human papillomaviruses

Författare och institution:
Baback Gharizadeh (-); Biying Zheng (-); Michael Akhras (-); Mehran Ghaderi (-); Olufisayo Jejelowo (-); Björn Strander (Institutionen för kliniska vetenskaper, sektionen för kvinnors och barns hälsa); Pål Nyrén (-); Keng-Ling Wallin (-); Nader Pourmand (-)
Publicerad i:
Mol Cell Probes, 20 ( 3-4 ) s. 230-8
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Despite the various technologies in place for genotyping human papillomaviruses (HPV), clinical use and clinical research demand a method that is fast, more reliable and cost-effective. The technology described here represents a breakthrough development in that direction. By combining the method of multiple sequencing primers with DNA sequencing, we have developed a rapid assay for genotyping HPV that relies on the identification of a single, type-specific 'sentinel' base. As described here, the prototype assay has been developed to recognize the 12 most high-risk HPV types (HPV-16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58 and 59) and is capable of recognizing and simultaneously genotyping multiple HPV co-infections. By providing sequence information on multiple HPV infections, this method eliminates the need for labor- and cost-intensive PCR cloning. These proof-of-concept studies establish the assay to be accurate, reliable, rapid, flexible, and cost-effective, providing evidence of the feasibility this technique for use in clinical settings.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Cervical Intraepithelial Neoplasia/blood/diagnosis/*virology, DNA Primers/biosynthesis/*genetics, DNA Probes, HPV/genetics, DNA, Viral/blood/genetics, Female, Genotype, Humans, Papillomaviridae/classification/*genetics/isolation & purification, Papillomavirus Infections/blood/diagnosis/*virology, Polymerase Chain Reaction, Risk Factors, Sensitivity and Specificity
Postens nummer:
Posten skapad:
2007-10-29 10:33

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