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Göteborgs universitets publikationer

Cerebrospinal fluid biomarkers in experimental spinal nerve root injury

Författare och institution:
M. Cornefjord (-); F. Nyberg (-); L. Rosengren (-); Helena Brisby (Institutionen för de kirurgiska disciplinerna, Avdelningen för ortopedi)
Publicerad i:
Spine, 29 ( 17 ) s. 1862-8
1528-1159 (Electronic)
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
STUDY DESIGN: Cerebrospinal fluid biomarkers were evaluated in a setup using established pig models to mimic clinical disc herniation. OBJECTIVES: To investigate biomarkers for nerve tissue injury, inflammation, and pain in cerebrospinal fluid after mechanical compression and/or nucleus pulposus application to spinal nerve roots. SUMMARY OF BACKGROUND DATA: The association between mechanical compression, biochemical effects of nucleus pulposus, and nerve root injury in degenerative disc disorders is incompletely investigated. METHODS: The unilateral S1 nerve root was exposed in 20 pigs. The animals were divided into four groups (n = 5 each): 1) slow-onset mechanical compression with an ameroid constrictor; 2) autologous nucleus pulposus application; 3) mechanical compression plus nucleus pulposus; and 4) sham operation. After 1 week, 6 mL of cerebrospinal fluid was collected, and four structural nerve proteins, neurofilaments, S-100, glial fibrillary acidic protein, neuron-specific enolase, the proinflammatory cytokine interleukin-8, the neurotransmitter nociceptin, and substance P endopeptidase activity were analyzed using immunoassays. RESULTS: The concentration of neurofilament was increased in the mechanical compression group (17.0 microg/L +/- 5.0) and in the mechanical compression plus nucleus pulposus group (19.8 +/- 12.1 microg/L) compared with the sham group (0.9 +/- 0.9 microg/L) and the nucleus pulposus group (0.4 +/- 0.1 microg/L) (P < 0.01 for both). The concentration of nociceptin was increased significantly in the mechanical compression group (24.0 +/- 8.6 fm/mL) and in the mechanical compression plus nucleus pulposus group (31.2 +/- 6.6 fm/mL) compared with the sham group (7.0 +/- 1.3 fm/mL) (P < 0.05 and P < 0.01, respectively). A correlation was found between concentrations of neurofilament and nociceptin (r = 0.50, P < 0.05). There were no intergroup differences regarding glial fibrillary acidic protein, neuron-specific enolase, S-100, interleukin-8, or substance P endopeptidase activity. CONCLUSIONS: The present study demonstrates increased concentrations of neurofilament and nociceptin in cerebrospinal fluid after nerve root compression. A simultaneous application of nucleus pulposus did not increase the response.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin ->
Kirurgi ->
Animals, Biological Markers, Cerebrospinal Fluid Proteins/*analysis, Glial Fibrillary Acidic Protein/cerebrospinal fluid, Interleukin-8/cerebrospinal fluid, Intervertebral Disk/*chemistry, Lumbar Vertebrae, Metalloendopeptidases/cerebrospinal fluid, Nerve Tissue Proteins/*cerebrospinal fluid, Neuralgia/*cerebrospinal fluid/etiology, Neurofilament Proteins/cerebrospinal fluid, Opioid Peptides/cerebrospinal fluid, Phosphopyruvate Hydratase/cerebrospinal fluid, Pressure/adverse effects, S100 Proteins/cerebrospinal fluid, Spinal Nerve Roots/drug effects/*injuries, Stress, Mechanical, Swine, Tissue Extracts/pharmacology/*toxicity
Postens nummer:
Posten skapad:
2007-10-11 13:54

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