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Göteborgs universitets publikationer

Helicobacter pylori-specific CD4+ T cells home to and accumulate in the human Helicobacter pylori-infected gastric mucosa

Författare och institution:
Anna Lundgren (Institutionen för medicinsk mikrobiologi och immunologi); C. Trollmo (-); Anders Edebo (Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi); Ann-Mari Svennerholm (Institutionen för medicinsk mikrobiologi och immunologi); Samuel B Lundin (Institutionen för medicinsk mikrobiologi och immunologi)
Publicerad i:
Infect Immun, 73 ( 9 ) s. 5612-9
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Helicobacter pylori infects the stomach and duodenal mucosa. T cells are important components of the H. pylori-induced immune response, but little is currently known about how these cells are recruited to the infected mucosa. Here, we have characterized stomach and duodenal T cells isolated from H. pylori-infected and noninfected subjects with regard to subtype, expression of homing and chemokine receptors, and in vitro reactivity to H. pylori antigens. Higher numbers of CD4(+) but similar numbers of CD8(+) lamina propria T cells were isolated from stomach biopsies from H. pylori-positive compared to H. pylori-negative individuals. CD4(+) T cells from infected stomach expressed increased levels of the homing receptor L-selectin and the chemokine receptor CCR4 compared to CD4(+) T cells from uninfected stomach. Infected stomach mucosa also contained increased levels of the CCR4 chemokine ligand MDC/CCL22. In contrast, comparable numbers of CD4(+) T cells with similar receptor expression were isolated from the duodenum of H. pylori-positive and H. pylori-negative individuals. In vitro proliferation of mucosal T cells was strongly enhanced by the addition of interleukin-2 (IL-2) and IL-7 to the cell cultures. Using this approach, H. pylori-specific T-cell responses were detected in stomach CD4(+) T cells from H. pylori-positive but not H. pylori-negative individuals. Duodenal T cells from only a few individuals responded to H. pylori stimulation, and the responsiveness was not restricted to H. pylori-positive individuals, suggesting limited H. pylori specificity in the duodenum and possible cross-reactivity with antigens from other bacteria in this compartment. In conclusion, these results suggest that H. pylori-specific CD4(+) T cells preferentially home to and accumulate in the infected stomach and that L-selectin and CCR4/MDC are important for this recruitment.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Adult, CD4-Positive T-Lymphocytes/*immunology/microbiology, Cell Aggregation/immunology, Cell Movement/*immunology, Chemokines/biosynthesis, Female, Gastric Mucosa/*immunology/microbiology, Helicobacter Infections/*immunology, Helicobacter pylori/*immunology, Humans, Male, Middle Aged, Receptors, CCR5/biosynthesis, Receptors, Chemokine/biosynthesis, Receptors, Lymphocyte Homing/biosynthesis
Postens nummer:
Posten skapad:
2007-10-10 14:37
Posten ändrad:
2011-01-20 10:00

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