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Hormonal regulation of mummy is needed for apical extracellular matrix formation and epithelial morphogenesis in Drosophila.

Författare och institution:
Anna Tonning (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Sigrun Helms (-); Heinz Schwarz (-); Anne Uv (Institutionen för biomedicin, avdelningen för medicinsk genetik och klinisk genetik); Bernard Moussian (-)
Publicerad i:
Development (Cambridge, England), 133 ( 2 ) s. 331-41
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Many epithelia produce apical extracellular matrices (aECM) that are crucial for organ morphogenesis or physiology. Apical ECM formation relies on coordinated synthesis and modification of constituting components, to enable their subcellular targeting and extracellular assembly into functional matrices. The exoskeleton of Drosophila, the cuticle, is a stratified aECM containing ordered chitin polysaccharide lamellae and proteinaceous layers, and is suited for studies of molecular functions needed for aECM assembly. Here, we show that Drosophila mummy (mmy) mutants display defects in epithelial organisation in conjunction with aberrant deposition of the cuticle and an apical matrix needed for tracheal tubulogenesis. We find that mmy encodes the UDP-N-acetylglucosamine pyrophosphorylase, which catalyses the production of UDP-N-acetylglucosamine, an obligate substrate for chitin synthases as well as for protein glycosylation and GPI-anchor formation. Consequently, in mmy mutants GlcNAc-groups including chitin are severely reduced and modification and subcellular localisation of proteins designated for extracellular space is defective. Moreover, mmy expression is selectively upregulated in epithelia at the time they actively deposit aECM, and is altered by the moulting hormone 20-Hydroxyecdysone, suggesting that mmy is part of a developmental genetic programme to promote aECM formation.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Amino Acid Sequence, Animals, Chitin, metabolism, Drosophila, genetics, growth & development, metabolism, Drosophila Proteins, genetics, metabolism, Ecdysterone, biosynthesis, Epithelium, growth & development, metabolism, ultrastructure, Extracellular Matrix, metabolism, Gene Expression Regulation, Developmental, Gene Expression Regulation, Enzymologic, Genes, Insect, Insect Hormones, metabolism, Microscopy, Electron, Molecular Sequence Data, Morphogenesis, Mutation, Nucleotidyltransferases, genetics, metabolism, Protein Processing, Post-Translational, Sequence Homology, Amino Acid
Postens nummer:
Posten skapad:
2007-10-08 14:11
Posten ändrad:
2011-01-20 10:00

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