transparent gif


Ej inloggad.

Göteborgs universitets publikationer

The C-type lectin MGL expressed by dendritic cells detects glycan changes on MUC1 in colon carcinoma

Författare och institution:
Eirikur Saeland (-); Sandra van Vliet (-); Malin Bäckström (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Venice CM van den Berg (-); Teunis BH Geijtenbeek (-); Gerritt A Meijer (-); Yvette van Kooyk (-)
Publicerad i:
Cancer Immunol Immunother, 56 ( 8 ) s. 1225-1236
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The epithelial mucin MUC1 is a high molecular weight membrane glycoprotein frequently overexpressed and aberrantly glycosylated in adenocarcinoma. Mucins normally contain high amounts of O-linked carbohydrate structures that may influence immune reactions to this antigen. During malignant transformation, certain glyco-epitopes of MUC1, such as Tn-antigen, TF-antigen and their sialylated forms become exposed. The role of these glycan structures in tumor biology is unknown, but their presence is known to correlate with poor prognosis in several adenocarcinomas. We analyzed the potency of MUC1 containing Tn-antigens (MUC1-Tn) to target C-type lectins that function as carbohydrate recognition and uptake molecules on dendritic cells (DC). We identified the macrophage galactose type C-type lectin (MGL), expressed by both DC and macrophages, as the receptor for recognition and binding of MUC1-Tn. To validate the occurrence of MGL–MUC1 interactions in situ, we studied the binding of MGL to MUC1 in primary colon carcinoma tissue. Isolation of MUC1 out of colon carcinoma tissue showed strong binding activity to MGL. Interestingly, MGL binding to MUC1 was highly correlated to binding by the lectin Helix pomatia agglutinin (HPA), which is associated with poor prognosis in colorectal cancer. The detection of MGL positive cells in situ at the tumor site together with the modified glycosylation status of MUC1 to target MGL on DC suggests that MGL positive antigen presenting cells may play a role in tumor progression.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Mikrobiologi inom det medicinska området
Medicinska grundvetenskaper ->
Annan medicinsk grundvetenskap ->
MUC1, lectin, dendritic cells
Postens nummer:
Posten skapad:
2007-10-01 14:18
Posten ändrad:
2010-01-26 12:48

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007