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Göteborgs universitets publikationer

CD4+ CD25+ FOXP3+ regulatory T cells from human thymus and cord blood suppress antigen-specific T cell responses

Författare och institution:
Kajsa Wing (Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning); Pia Larsson (Institutionen för invärtesmedicin); Kerstin Sandström (Institutionen för kvinnors och barns hälsa); Samuel B Lundin (Institutionen för medicinsk mikrobiologi och immunologi); Elisabeth Suri-Payer (-); Anna Rudin (Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning)
Publicerad i:
Immunology, 115 ( 4 ) s. 516-25
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Activation of self-reactive T cells in healthy adults is prevented by the presence of autoantigen-specific CD4+CD25+ regulatory T cells (CD25+ Treg). To explore the functional development of autoantigen-reactive CD25+ Treg in humans we investigated if thymic CD25+ Treg from children aged 2 months to 11 years and cord blood CD25+ Treg are able to suppress proliferation and cytokine production induced by specific antigens. While CD4+CD25- thymocytes proliferated in response to myelin oligodendrocyte glycoprotein (MOG), tetanus toxoid and beta-lactoglobulin, suppression of proliferation was not detected after the addition of thymic CD25+ Treg. However, CD25+ Treg inhibited interferon (IFN)-gamma production induced by MOG, which indicates that MOG-reactive CD25+ Treg are present in the thymus. In contrast, cord blood CD25+ Treg suppressed both proliferation and cytokine production induced by MOG. Both cord blood and thymic CD25+ Treg expressed FOXP3 mRNA. However, FOXP3 expression was lower in cord blood than in thymic CD25+ T cells. Further characterization of cord blood CD25+ T cells revealed that FOXP3 was highly expressed by CD25+CD45RA+ cells while CD25+CD45RA- cells contained twofold less FOXP3, which may explain the lower expression level of FOXP3 in cord blood CD25+ T cells compared to thymic CD25+ T cells. In conclusion, our data demonstrate that low numbers of MOG-reactive functional CD25+ Treg are present in normal thymus, but that the suppressive ability of the cells is broader in cord blood. This suggests that the CD25+ Treg may be further matured in the periphery after being exported from the thymus.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Immunologi inom det medicinska området ->
Antigens, Surface/immunology, CD4-Positive T-Lymphocytes/immunology, Cell Division/immunology, Cells, Cultured, Child, Child, Preschool, DNA-Binding Proteins/*immunology, Epitopes/immunology, Fetal Blood/*immunology, Forkhead Transcription Factors, Humans, Infant, Infant, Newborn, Interferon Type II/immunology, Interleukins/immunology, Myelin-Associated Glycoprotein/immunology, RNA, Messenger/analysis, Receptors, Interleukin-2/*immunology, T-Lymphocytes/*immunology, Thymus Gland/*immunology
Postens nummer:
Posten skapad:
2007-09-28 14:18
Posten ändrad:
2010-01-26 11:05

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