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Göteborgs universitets publikationer

High local concentrations and effects on differentiation implicate interleukin-6 as a paracrine regulator

Författare och institution:
Victoria Rotter Sopasakis (Institutionen för invärtesmedicin, Avdelningen för internmedicin); Madelene Sandqvist (Institutionen för invärtesmedicin, Avdelningen för internmedicin); Birgit Gustafson (Institutionen för invärtesmedicin, Avdelningen för internmedicin); Ann Hammarstedt (Institutionen för invärtesmedicin, Avdelningen för internmedicin); M. Schmelz (-); Xiao Lin Yang (Institutionen för invärtesmedicin, Avdelningen för internmedicin); Per-Anders Jansson (Institutionen för invärtesmedicin, Avdelningen för internmedicin); Ulf Smith (Institutionen för invärtesmedicin, Avdelningen för internmedicin)
Publicerad i:
Obes Res, 12 ( 3 ) s. 454-60
1071-7323 (Print)
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
OBJECTIVE: To examine the possibility that interleukin-6 (IL-6) can act as a paracrine regulator in adipose tissue by examining effects on adipogenic genes and measuring interstitial IL-6 concentrations in situ. RESEARCH METHODS AND PROCEDURES: Circulating and interstitial IL-6 concentrations in abdominal and femoral adipose tissue were measured using the calibrated microdialysis technique in 20 healthy male subjects. The effects of adipose cell enlargement on gene expression and IL-6 secretion were examined, as well as the effect of IL-6 in vitro on gene expression of adiponectin and other markers of adipocyte differentiation. RESULTS: The IL-6 concentration in the interstitial fluid was approximately 100-fold higher than that in plasma, suggesting that IL-6 may be a paracrine regulator of adipose tissue. This was further supported by the finding that adding IL-6 in vitro at similar concentrations down-regulated the expression of adiponectin, aP2, and PPARgamma-2 in cultured human adipose tissue. In addition, gene expression and release of IL-6, both in vivo and in vitro, correlated with adipose cell size. DISCUSSION: These data suggest that IL-6 may be a paracrine regulator of adipose tissue. Furthermore, increased adipose tissue production of IL-6 after hypertrophic enlargement of the adipose cells may detrimentally affect systemic insulin action by inducing adipose tissue dysfunction with impaired differentiation of the pre-adipocytes and/or adipocytes and lower adiponectin.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Abdomen, Adipocytes/*cytology, Adiponectin, Adipose Tissue/chemistry/*physiology, Adult, Cell Differentiation/*drug effects/genetics, Cell Size, Femur, Gene Expression/drug effects, Humans, *Intercellular Signaling Peptides and Proteins, Interleukin-6/genetics/pharmacology/*physiology, Male, Microdialysis, Middle Aged, Proteins/genetics, Receptors, Cytoplasmic and Nuclear/genetics, Transcription Factors/genetics
Postens nummer:
Posten skapad:
2007-09-27 11:15
Posten ändrad:
2010-01-26 12:43

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