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Göteborgs universitets publikationer

Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen

Författare och institution:
Masafumi Tomaki (Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi); Lin-Ling Zhao (Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi); Margareta Sjöstrand (Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi); Anders Lindén (Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi); M. Ichinose (-); Jan Lötvall (Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi)
Publicerad i:
Pulmonary pharmacology & therapeutics, 15 ( 2 ) s. 161-8
1094-5539 (Print)
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Allergic inflammation is dominated by eosinophils. IL-3, IL-5, and GM-CSF are involved in production and activation of eosinophils. IL-5 has been reported to be crucial for the induction of airway eosinophilia. However, the contribution of IL-3 and GM-CSF to allergic airway inflammation remains to be determined. To address this issue, ovalbumin-sensitized Balb/c mice were repeatedly exposed to allergen via airway route. Animals were pretreated intraperitoneally with neutralising anti-IL-3, anti-IL-5 and/or anti-GM-CSF antibodies. Newly produced inflammatory cells were pulse-labelled with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU), which is incorporated into DNA during the cell mitosis. BAL and bone marrow cells were collected 24 h after the last allergen exposure, and differential cell counts and immunocytochemical detection of BrdU-labelled cells were performed. Anti-IL-5 strongly reduced both BAL and bone marrow eosinophilia, as well as the number of BrdU-positive BAL-granulocytes. In contrast, anti-IL-3 and anti-GM-CSF alone had little and no inhibitory effect on these responses, respectively. Even the combined treatment with anti-IL-3 and anti-GM-CSF showed only a non-significant tendency to attenuate these responses. These data suggest that the efficacy of treatments with anti-IL-3 and anti-GM-CSF is much weaker than that with anti-IL-5. IL-5 may be the preferred target to block eosinophilia in allergic diseases.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin ->
Lungmedicin och allergi
Allergens/*adverse effects, Animals, Antimetabolites/metabolism, Bone Marrow/*drug effects/metabolism, Bromodeoxyuridine/metabolism, Bronchoalveolar Lavage Fluid, Eosinophilia/*drug therapy/etiology/immunology, Granulocyte-Macrophage Colony-Stimulating Factor/*antagonists & inhibitors, Interleukin-3/*antagonists & inhibitors/immunology, Interleukin-5/*antagonists & inhibitors/immunology, Male, Mice, Mice, Inbred BALB C, Ovalbumin/adverse effects/immunology
Postens nummer:
Posten skapad:
2007-09-27 10:28
Posten ändrad:
2011-01-20 09:59

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