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Göteborgs universitets publikationer

Structure and function of the GTP binding protein Gtr1 and its role in phosphate transport in Saccharomyces cerevisiae

Författare och institution:
Jens O. Lagerstedt (Wallenberglaboratoriet); I. Reeve (-); J. C. Voss (-); B. L. Persson (-)
Publicerad i:
Biochemistry, 44 ( 2 ) s. 511-7
0006-2960 (Print)
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The Pho84 high-affinity phosphate permease is the primary phosphate transporter in the yeast Saccharomyces cerevisiae under phosphate-limiting conditions. The soluble G protein, Gtr1, has previously been suggested to be involved in the derepressible Pho84 phosphate uptake function. This idea was based on a displayed deletion phenotype of Deltagtr1 similar to the Deltapho84 phenotype. As of yet, the mode of interaction has not been described. The consequences of a deletion of gtr1 on in vivo Pho84 expression, trafficking and activity, and extracellular phosphatase activity were analyzed in strains synthesizing either Pho84-green fluorescent protein or Pho84-myc chimeras. The studies revealed a delayed response in Pho84-mediated phosphate uptake and extracellular phosphatase activity under phosphate-limiting conditions. EPR spectroscopic studies verified that the N-terminal G binding domain (residues 1-185) harbors the nucleotide responsive elements. In contrast, the spectra obtained for the C-terminal part (residues 186-310) displayed no evidence of conformational changes upon GTP addition.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Electron Spin Resonance Spectroscopy, Gene Deletion, Green Fluorescent Proteins/genetics/metabolism, Models, Molecular, Monomeric GTP-Binding Proteins/*chemistry/genetics/physiology, Phosphates/*metabolism, Protein Binding/genetics, Protein Conformation, Protein Transport/genetics, Proton-Phosphate Symporters/metabolism/physiology, Recombinant Fusion Proteins/metabolism, Saccharomyces cerevisiae, Proteins/*chemistry/genetics/metabolism/physiology, Structure-Activity Relationship, Thermodynamics
Postens nummer:
Posten skapad:
2007-09-20 10:13
Posten ändrad:
2007-09-20 10:52

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