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Göteborgs universitets publikationer

Lipopolysaccharide-induced granule mobilization and priming of the neutrophil response to Helicobacter pylori peptide Hp(2-20), which activates formyl peptide receptor-like 1

Författare och institution:
Johan Bylund (Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning); Anna Karlsson (Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning); F. Boulay (-); Claes Dahlgren (Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning)
Publicerad i:
Infect Immun, 70 ( 6 ) s. 2908-14
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The cecropin-like bactericidal peptide Hp(2-20) from Helicobacter pylori induces activation of the NADPH oxidase in human neutrophils via formyl peptide receptor-like 1 (FPRL1) (J. Bylund, T. Christophe, F. Boulay, T. Nystrom, A. Karlsson, and C. Dahlgren, Antimicrob. Agents Chemother. 45:1700-1704, 2001). Here we investigated the ability of bacterial lipopolysaccharide (LPS) to prime this response. Neutrophils treated with LPS for 30 min at 37 degrees C produced substantially more superoxide anion than control cells upon stimulation with Hp(2-20). Hence, LPS primed the cells for subsequent stimulation through FPRL1. To study the molecular background of this priming phenomenon, we measured the degrees of granule mobilization and concomitant receptor upregulation to the cell surface in LPS-treated cells. Exposure of complement receptors 1 and 3 as well as the formyl peptide receptor (FPR) was markedly increased after LPS treatment. Since approximately 60% of the gelatinase granules were mobilized while the specific granules were retained, we hypothesized that the gelatinase granules were potential stores of FPRL1. The presence of FPRL1 mainly in the gelatinase granules was confirmed by Western blotting of subcellular fractions of resting neutrophils. These results suggest that the mechanism behind the LPS-induced priming of FPRL1-mediated responses lies at the level of granule (receptor) mobilization.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Amino Acid Sequence, Anti-Bacterial Agents/*immunology, Bacterial Proteins/*immunology, Cells, Cultured, Gelatinases/immunology, Helicobacter pylori/*immunology, Humans, Lipopolysaccharides/*immunology/pharmacology, Macrophage-1 Antigen/immunology, Molecular Sequence Data, Neutrophils/drug effects/*immunology, Organelles, Peptide Fragments/*immunology, Peptides/immunology, Receptors, Complement 3b/immunology, Receptors, Formyl Peptide, Receptors, Immunologic/*immunology, *Receptors, Lipoxin, Receptors, Peptide/*immunology, Subcellular Fractions, Superoxides
Postens nummer:
Posten skapad:
2007-09-19 16:14
Posten ändrad:
2010-01-26 12:48

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