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Göteborgs universitets publikationer

Phenotypic and functional characterization of human CD25+ B cells

Författare och institution:
Mikael Brisslert (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Maria Bokarewa (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Pia Larsson (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); K. Wing (-); Vincent Collins (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Andrej Tarkowski (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning)
Publicerad i:
Immunology, 117 ( 4 ) s. 548-57
0019-2805 (Print)
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
We demonstrate that humans have a phenotypically and functionally distinct subset of B lymphocytes that express the interleukin (IL)-2 receptor (IL-2R) alpha-chain, cluster of differentiation (CD) 25. We found that one-third of the circulating CD20+ B cells expressed CD25 and, using fluorescence-activated cell sorter (FACS) analysis, that these cells were significantly larger and more granulated than B cells not expressing CD25. The simultaneous expression of the other two subunits (CD122 and CD132) and the proliferative responses of cells expressing CD25 to IL-2 suggested that, in addition to CD25, functional IL-2 receptors were expressed on this cell population. CD25 expression on B cells was selectively up-regulated by Toll-like receptor 2 (TLR2), TLR4, and TLR9 ligands but not by a TLR3 ligand or Epstein-Barr virus (EBV) stimulation. Blockade of the nuclear factor (NF)-kappaB pathway completely abolished CD25 up-regulation by these B cells. Interestingly, CD25+ B cells expressed significantly higher levels of surface immunoglobulins but lacked the ability to secrete immunoglobulin (Ig), as compared with CD25- B cells. Furthermore, CD25+ B cells performed significantly better as antigen-presenting cells in allogeneic mixed lymphocyte reactions (MLR), which may be a result of their expression of high levels of the costimulatory molecules CD27 and CD80. Finally, blocking of CD25 on B cells led to an almost total abrogation of MLR. Our results indicate that CD25+ B cells have distinct phenotypic and functional properties, including the ability to contribute to antigen presentation, which is linked to their expression of CD25. Finally, the differential regulation of CD25 expression via selective TLR ligands suggests a role for CD25+ B cells in bridging innate and acquired immune responses.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Adult, Antigen Presentation/immunology, B-Lymphocyte Subsets/*immunology, Cell Separation/methods, Flow Cytometry/methods, Herpesvirus 4, Human/immunology, Humans, Immunoglobulins/biosynthesis, Immunophenotyping/methods, Interleukin-2/immunology, Ligands, Lymphocyte Culture Test, Mixed, Mitogens/immunology, Receptors, Interleukin-2/*blood, Toll-Like Receptors/immunology
Postens nummer:
Posten skapad:
2007-07-03 09:39
Posten ändrad:
2011-01-20 10:00

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