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Göteborgs universitets publikationer

Increased expression of leukocyte Ig-like receptor-1 and activating role of UL18 in the response to cytomegalovirus infection

Författare och institution:
C. S. Wagner (-); Gerdt C. Riise (Institutionen för medicin, avdelningen för invärtesmedicin); Tomas Bergström (Institutionen för biomedicin, avdelningen för infektionssjukdomar); K. Kärre (-); E. Carbone (-); L. Berg (-)
Publicerad i:
J Immunol, 178 ( 6 ) s. 3536-43
0022-1767 (Print)
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
NK and T cells are important for combating CMV infection. Some NK and T cells express leukocyte Ig-like receptor-1 (LIR-1), an inhibitory receptor recognizing MHC class I and the CMV-encoded homolog UL18. We previously demonstrated an early increase in LIR-1-expressing blood lymphocytes in lung-transplanted patients later developing CMV disease. We now show that NK and T cells account for the observed LIR-1 augmentation. Coincubation of PBMC from CMV-seropositive donors with virus-infected lung fibroblasts led to a T cell-dependent secretion of IFN-gamma, produced mainly by LIR-1(+) T cells and by NK cells. Cytokine production during coculture with fibroblasts infected with virus containing the UL18 gene was augmented compared with the UL18 deletion virus, suggesting a stimulatory role for UL18. However, purified UL18Fc proteins inhibited IFN-gamma production of LIR-1(+) T cells. We propose that cytokine production in the transplant induces NK and T cells to express LIR-1, which may predispose to CMV disease by MHC/LIR-1-mediated suppression. Although the UL18/LIR-1 interaction could inhibit T cell responses, this unlikely plays a role in response to infected cells. Instead, our data point to an activating role for viral UL18 during infection, where indirect intracellular effects cannot be excluded.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin ->
Lungmedicin och allergi
Adult, Aged, Bystander Effect/immunology, Capsid Proteins/biosynthesis/*immunology, Cells, Cultured, Coculture Techniques, Cytomegalovirus/immunology, Cytomegalovirus Infections/etiology/*immunology/metabolism, Female, Fibroblasts/immunology/metabolism/virology, Histocompatibility Antigens Class I/immunology/metabolism, Humans, *Immune Tolerance, Immunity, Cellular, Killer Cells, Natural/immunology/metabolism, Lung Transplantation/*immunology, Male, Middle Aged, Receptors, Immunologic/biosynthesis/*immunology, T-Lymphocytes/*immunology/metabolism
Postens nummer:
Posten skapad:
2007-06-19 11:32
Posten ändrad:
2011-01-20 10:00

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