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Göteborgs universitets publikationer

Nucleus pulposus-induced apoptosis in dorsal root ganglion following experimental disc herniation in rats

Författare och institution:
Y. Murata (-); Ulf Nannmark (Institutionen för anatomi och cellbiologi); Björn Rydevik (Institutionen för kliniska vetenskaper, sektionen för anestesi, biomaterial och ortopedi); K. Takahashi (-); Kjell Olmarker (Institutionen för kliniska vetenskaper, sektionen för anestesi, biomaterial och ortopedi)
Publicerad i:
Spine, 31 ( 4 ) s. 382-90
1528-1159 (Electronic)
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
STUDY DESIGN: The mechanisms behind the formation of a characteristic tissue reaction at the surface of the dorsal root ganglion (DRG) exposed to nucleus pulposus was studied with special reference to apoptosis using electron microscopy and immunohistochemistry in rats. OBJECTIVES: To study the mechanism of the characteristic tissue reaction at the surface of the DRG exposed to nucleus pulposus. SUMMARY OF BACKGROUND DATA: Recently, it was observed that local application of nucleus pulposus may induce a characteristic tissue reaction at the surface of the DRG. This change occurred as early as 1 day after the application of nucleus pulposus. METHODS.: Herniation of nucleus pulposus was created in the L4-L5 disc in rats. The L4 DRG were resected 3 and 24 hours after surgery. The sections of the specimens were observed using light and electron microscopy. The sections were processed for immunohistochemistry using antibodies to single-stranded DNA (ssDNA), caspase 3, and tumor necrosis factor alpha (TNF). RESULTS: There were typical changes of the cell nuclei observed by light and electron microscopy, especially those of the small-sized cells, in the DRG 24 hours after application of nucleus pulposus, indicating the presence of apoptosis. The presence of ssDNA, caspase 3, and TNF further enhanced the impression that there was apoptosis in the DRG. Nucleus pulposus induced apoptosis in the DRG at the site of application within as little as 24 hours. CONCLUSIONS: Nucleus pulposus herniated from the disc induced apoptosis in at the surface of the DRG exposed to nucleus pulpous as early as 24 hours after exposure.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin ->
Kirurgi ->
Animals, *Apoptosis, Biological Markers/metabolism, Caspase 3, Caspases/metabolism, Cell Nucleus/ultrastructure, DNA, Single-Stranded/analysis, Disease Models, Animal, Ganglia, Spinal/chemistry/metabolism/*pathology, Immunohistochemistry, Intervertebral Disk/metabolism/*pathology/surgery, Intervertebral Disk Displacement/complications/*pathology, Laminectomy, Lumbar Vertebrae, Rats, Sprains and Strains, Tumor Necrosis Factor-alpha/metabolism
Postens nummer:
Posten skapad:
2007-05-15 14:24
Posten ändrad:
2011-01-20 09:59

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