transparent gif


Ej inloggad.

Göteborgs universitets publikationer

Localization of human neutrophil interleukin-8 (CXCL-8) to organelle(s) distinct from the classical granules and secretory vesicles

Författare och institution:
Sara Pellmé (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); M. Morgelin (-); H. Tapper (-); Ulf-Henrik Mellqvist (Institutionen för medicin, avdelningen för invärtesmedicin); Claes Dahlgren (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Anna Karlsson (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning)
Publicerad i:
J Leukoc Biol, 79 ( 3 ) s. 564-73
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Mature human neutrophils contain small amounts of interleukin-8 [CXC chemokine ligand 8 (CXCL-8)], which upon proinflammatory activation, increases significantly. It has been suggested that the CXCL-8 content of resting human neutrophils is stored in the secretory vesicles. Here, we have used a fractionation technique, which allows isolation of these vesicles, and we find that CXCL-8 neither colocalizes with the secretory vesicles nor with markers of any of the classical neutrophil granules. To increase resolution in the system, we induced CXCL-8 production by lipopolysaccharide. After 8 h of stimulation, CXCL-8 was visualized within the cell using immunoelectron microscopy. The images revealed CXCL-8-containing stuctures resembling neutrophil granules, and these were distinct from all known neutrophil organelles, as shown by double immunostaining. Further, the CXCL-8 organelle was present in nonstimulated neutrophil cytoplasts, entities lacking all other known granules and secretory vesicles. Upon fractionation of the cytoplasts, CXCL-8 was found to partly cofractionate with calnexin, a marker for endoplasmic reticulum (ER). Thus, part of CXCL-8 may be localized to the ER or ER-like structures in the neutrophil.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Biological Markers/metabolism, Calnexin/metabolism, Cells, Cultured, Cytoplasmic Granules/*metabolism/secretion/ultrastructure, Endoplasmic Reticulum/*metabolism/secretion/ultrastructure, Histocytochemistry, Humans, Interleukin-8/immunology/*metabolism/secretion, Intracellular Membranes/drug effects/metabolism/secretion, Lipopolysaccharides, Microscopy, Electron, Transmission, Microscopy, Immunoelectron, Neutrophils/*metabolism/secretion/ultrastructure, Organelles/*metabolism/secretion/ultrastructure, Research Support, Non-U.S. Gov't, Secretory Vesicles/*metabolism/secretion/ultrastructure, Subcellular Fractions
Postens nummer:
Posten skapad:
2007-04-05 11:18
Posten ändrad:
2011-01-20 09:59

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007