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Göteborgs universitets publikationer

Genomic buffering mitigates the effects of deleterious mutations in bacteria

Författare och institution:
Sophie Maisnier-Patin (-); John R. Roth (-); Åsa Fredriksson (Institutionen för cell- och molekylärbiologi); Thomas Nyström (Institutionen för cell- och molekylärbiologi); Otto G. Berg (-); Dan I. Andersson (-)
Publicerad i:
Nature Genetics, 37 ( 12 ) s. 1376-1379
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The relationship between the number of randomly accumulated mutations in a genome and fitness is a key parameter in evolutionary biology1, 2, 3, 4, 5. Mutations may interact such that their combined effect on fitness is additive (no epistasis), reinforced (synergistic epistasis) or mitigated (antagonistic epistasis). We measured the decrease in fitness caused by increasing mutation number in the bacterium Salmonella typhimurium using a regulated, error-prone DNA polymerase (polymerase IV, DinB). As mutations accumulated, fitness costs increased at a diminishing rate. This suggests that random mutations interact such that their combined effect on fitness is mitigated and that the genome is buffered against the fitness reduction caused by accumulated mutations. Levels of the heat shock chaperones DnaK and GroEL increased in lineages that had accumulated many mutations, and experimental overproduction of GroEL further increased the fitness of lineages containing deleterious mutations. These findings suggest that overexpression of chaperones contributes to antagonistic epistasis
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Biologiska vetenskaper ->
Biokemi och molekylärbiologi ->
Cell- och molekylärbiologi
Postens nummer:
Posten skapad:
2007-03-09 11:29
Posten ändrad:
2011-01-20 10:00

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