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Göteborgs universitets publikationer

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography TTOR J, 1990, FEBS LETTERS, V263, P269

Författare och institution:
P. Nogly (-); V. Panneels (-); G. Nelson (-); C. Gati (-); T. Kimura (-); C. Milne (-); D. Milathianaki (-); M. Kubo (-); W. T. Wu (-); C. Conrad (-); J. Coe (-); R. Bean (-); Y. Zhao (-); Petra Båth (Institutionen för kemi och molekylärbiologi); Robert Dods (Institutionen för kemi och molekylärbiologi); Rajiv Harimoorthy (Institutionen för kemi och molekylärbiologi); K. R. Beyerlein (-); J. Rheinberger (-); D. James (-); D. DePonte (-); C. F. Li (-); L. Sala (-); G. J. Williams (-); M. S. Hunter (-); J. E. Koglin (-); Peter Berntsen (Institutionen för kemi och molekylärbiologi); E. Nango (-); S. Iwata (-); H. N. Chapman (-); P. Fromme (-); M. Frank (-); R. Abela (-); S. Boutet (-); A. Barty (-); T. A. White (-); U. Weierstall (-); J. Spence (-); Richard Neutze (Institutionen för kemi och molekylärbiologi); G. Schertler (-); J. Standfuss (-)
Publicerad i:
Nature Communications, 7
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 angstrom resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Biologiska vetenskaper
x-ray-diffraction, protein-coupled receptors, coherent-light source, femtosecond crystallography, membrane-proteins, radiation-damage, macromolecular crystallography, structural alterations, photocycle, kinetics, bacteriorhodopsin, Science & Technology - Other Topics
Postens nummer:
Posten skapad:
2016-09-20 09:21

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