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Systems Level Analysis of the Yeast Osmo-Stat

Författare och institution:
Soheil Rastgou Talemi (-); Carl Fredrik Tiger (Institutionen för kemi och molekylärbiologi); Mikael Andersson (Institutionen för kemi och molekylärbiologi); Roja Babazadeh (Institutionen för kemi och molekylärbiologi); Niek Welkenhuysen (Institutionen för kemi och molekylärbiologi); Edda Klipp (-); Stefan Hohmann (Institutionen för kemi och molekylärbiologi & Institutionen för biologi och bioteknik, Chalmers); Joerg Schaber (-)
Publicerad i:
Scientific Reports, 6 s. Article Number: 30950
Artikel, refereegranskad vetenskaplig
Fulltextlänk (lokalt arkiv):
Sammanfattning (abstract):
Adaptation is an important property of living organisms enabling them to cope with environmental stress and maintaining homeostasis. Adaptation is mediated by signaling pathways responding to different stimuli. Those signaling pathways might communicate in order to orchestrate the cellular response to multiple simultaneous stimuli, a phenomenon called crosstalk. Here, we investigate possible mechanisms of crosstalk between the High Osmolarity Glycerol (HOG) and the Cell Wall Integrity (CWI) pathways in yeast, which mediate adaptation to hyper- and hypo-osmotic challenges, respectively. We combine ensemble modeling with experimental investigations to test in quantitative terms different hypotheses about the crosstalk of the HOG and the CWI pathways. Our analyses indicate that for the conditions studied i) the CWI pathway activation employs an adaptive mechanism with a variable volume-dependent threshold, in contrast to the HOG pathway, whose activation relies on a fixed volume-dependent threshold, ii) there is no or little direct crosstalk between the HOG and CWI pathways, and iii) its mainly the HOG alone mediating adaptation of cellular osmotic pressure for both hyper- as well as hypo-osmotic stress. Thus, by iteratively combining mathematical modeling with experimentation we achieved a better understanding of regulatory mechanisms of yeast osmo-homeostasis and formulated new hypotheses about osmo-sensing.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Biologiska vetenskaper
Eukaryotic unicellular organism biology systems biology of the control of cell growth and proliferation (UNICELLSYS ) (EC/FP7/201142) Mer information
Postens nummer:
Posten skapad:
2016-09-16 10:59
Posten ändrad:
2016-09-23 13:52

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