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Concentration-Induced Association in a Protein System Caused by a Highly Directional Patch Attraction

Författare och institution:
Weimin Li (-); Björn A. Persson (-); Mikael Lund (-); Johan Bergenholtz (Institutionen för kemi och molekylärbiologi); Malin Zackrisson Oskolkova (-)
Publicerad i:
Journal of Physical Chemistry B, 120 s. 8953-8959
ISSN:
1520-6106
E-ISSN:
1520-5207
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Self-association of the protein lactoferrin is studied in solution using small-angle X-ray scattering techniques. Effective static structure factors have been shown to exhibit either a monotonic or a nonmonotonic dependence on protein concentration in the small wavevector limit, depending on salt concentration. The behavior correlates with a nonmonotonic dependence of the second virial coefficient on salt concentration, such that a maximum appears in the structure factor at a low protein concentration when the second virial coefficient is negative and close to a minimum. The results are interpreted in terms of an integral equation theory with explicit dimers, formulated by Wertheim, which provides a consistent framework able to explain the behavior in terms of a monomer–dimer equilibrium that appears because of a highly directional patch attraction. Short attraction ranges preclude trimer formation, which explains why the protein system behaves as if it were subject to a concentration-dependent isotropic protein–protein attraction. Superimposing an isotropic interaction, comprising screened Coulomb repulsion and van der Waals attraction, on the patch attraction allows for a semiquantitative modeling of the complete transition pathway from monomers in the dilute limit to monomer–dimer systems at somewhat higher protein concentrations.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
NATURVETENSKAP ->
Kemi ->
Fysikalisk kemi ->
Biofysikalisk kemi
NATURVETENSKAP ->
Kemi ->
Fysikalisk kemi ->
Yt- och kolloidkemi
NATURVETENSKAP ->
Kemi ->
Teoretisk kemi ->
Statistisk mekanik
Nyckelord:
protein interactions, SAXS, light scattering, integral equation theory, structure factor
Postens nummer:
241419
Posten skapad:
2016-09-07 13:04

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