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Göteborgs universitets publikationer

Reference measurement procedure for CSF Aβ1-42 and the CSF Aβ1-42 /Aβ1-40 ratio - a cross-validation study against Amyloid PET.

Författare och institution:
Josef Pannee (Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi); Erik Portelius (Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi); Lennart Minthon (-); Johan Gobom (Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi); Ulf Andreasson (Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi); Henrik Zetterberg (Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi); Oskar Hansson (-); Kaj Blennow (Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi)
Publicerad i:
Journal of neurochemistry,
ISSN:
1471-4159
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
A clinical diagnosis of Alzheimer's disease (AD) is currently made on the basis of results from cognitive tests in combination with medical history and general clinical evaluation, but the peptide amyloid-beta (Aβ) in cerebrospinal fluid (CSF) is increasingly used as a biomarker for amyloid pathology in clinical trials and in recently proposed revised clinical criteria for AD. Recent analytical developments have resulted in mass spectrometry (MS) reference measurement procedures (RMP) for absolute quantification of Aβ1-42 in CSF. The CSF Aβ1-42 /Aβ1-40 ratio has been suggested to improve the detection of cerebral amyloid deposition, by compensating for inter-individual variations in total Aβ production. Our aim was to cross-validate the RMP as well as the Aβ1-42 /Aβ1-40 and Aβ1-42 /Aβ1-38 ratios in CSF, measured by high resolution MS, with the cortical level of Aβ fibrils as measured by amyloid ((18) F-flutemetamol) positron emission tomography (PET). We included 100 non-demented patients with cognitive symptoms from the Swedish BioFINDER study, all of whom had undergone both lumbar puncture and (18) F-flutemetamol PET. Comparing CSF Aβ1-42 concentrations with (18) F-flutemetamol PET showed high concordance with an area under the receiver operating characteristic (ROC) curve of 0.85 and a sensitivity and specificity of 82% and 81% respectively. The ratio of Aβ1-42 /Aβ1-40 or Aβ1-42 /Aβ1-38 significantly improved concordance with an area under the ROC curve of 0.95 and a sensitivity and specificity of 96% and 91% respectively. These results show that the CSF Aβ1-42 /Aβ1-40 and Aβ1-42 /Aβ1-38 ratios using the described MS method are strongly associated with cortical Aβ fibrils measured by (18) F-flutemetamol PET. This article is protected by copyright. All rights reserved.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Medicinska grundvetenskaper ->
Neurovetenskaper ->
Neurokemi
Postens nummer:
241387
Posten skapad:
2016-09-06 18:21

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