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Göteborgs universitets publikationer

THE NEUTROPHIL RESPONSE INDUCED BY AN AGONIST FOR THE FREE FATTY ACID RECEPTOR 2 (FFA2R/GPR43) IS PRIMED BY TNF-α AND BY RECEPTOR UNCOUPLING FROM THE CYTOSKELETON BUT ATTENUATED BY TISSUE RECRUITMENT.

Författare och institution:
Lena Björkman (Institutionen för medicin); Jonas Mårtensson (Institutionen för medicin); Malene Winther (Institutionen för medicin); Michael Gabl (Institutionen för medicin); André Holdfeldt (Institutionen för nationalekonomi med statistik); Martin Uhrbom (-); Johan Bylund (Institutionen för odontologi); Anders Højgaard Hansen (-); Sunil K Pandey (-); Trond Ulven (-); Huamei Forsman (Institutionen för medicin); Claes Dahlgren (Institutionen för medicin)
Publicerad i:
Molecular and cellular biology,
ISSN:
1098-5549
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Ligands with improved potency and selectivity for free fatty acid receptor 2 (FFA2R) have become available and we here characterize the neutrophil responses induced by one such agonist (Cmp1) and one antagonist (CATPB). Cmp1 triggered an increase in the cytosolic concentration of Ca(2+) and the neutrophils were then desensitized to Cmp1 and to acetate, a naturally occurring FFA2R agonist. The antagonist CATPB selectively inhibited responses induced by Cmp1 or acetate.The activated FFA2R induced superoxide anion secretion at a low level in naïve blood neutrophils. This response was largely increased by TNF-α in a process associated with a recruitment of easily mobilizable granules, but neutrophils recruited to an aseptic inflammation in vivo, were non-responding. Superoxide production induced by Cmp1 was increased in latrunculin A treated neutrophils, but no reactivation of desensitized FFA2R was induced by this drug, suggesting that the cytoskeleton is not directly involved in terminating the response. The functional and regulatory differences between the receptors that recognize short chain fatty acids and formylated peptides, respectively, imply different roles of these receptors in the orchestration of inflammation and confirm the usefulness of selective FFA2R agonist and antagonist as tools for the exploration of the precise role of the FFA2R.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Medicinska grundvetenskaper
MEDICIN OCH HÄLSOVETENSKAP ->
Klinisk medicin
Postens nummer:
241214
Posten skapad:
2016-09-02 16:06

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