transparent gif


Ej inloggad.

Göteborgs universitets publikationer

Antibiotic resistance in the environment: a contribution from metagenomic studies

Författare och institution:
Johan Bengtsson-Palme (Institutionen för biomedicin, avdelningen för infektionssjukdomar)
Antal sidor:
University of Gothenburg
Datum för examination:
Tidpunkt för examination:
Arvid Carlsson, Medicinaregatan 3
Thomas Berendonk
Sammanfattning (abstract):
Antibiotic resistance accounts for hundreds of thousands of deaths annually, and its projected increase has made the WHO recognize it as a major global health threat. In the last decade, evidence has mounted suggesting that the environment plays an important role in the progression of resistance. The external environment acts as a source of resistance genes for human pathogens, but is also an important dissemination route allowing the spread of resistant bacteria between different environments and human populations. In this thesis, large-scale DNA sequencing techniques are used to gain a better understanding of the risks associated with environmental antibiotic resistance. A key task in this process is the quantification of the number of antibiotic resistance genes in different environments using metagenomics. However, equally important is to put this information into a larger perspective, by including, for example, taxonomic data, concentrations of antibiotics present, and the genomic contexts of identified resistance genes. This thesis presents a software tool – Metaxa2 – for improved taxonomic analysis of shotgun metagenomic data, which is shown to give more accurate taxonomic classifications of short read data than other tools (Paper I). It also provides theoretically predicted no-effect concentrations for 111 antibiotics (Paper II), and experimentally determined minimal selective concentrations for tetracycline (Paper III). Furthermore, resistance genes are quantified in two environments suggested to pose selective conditions for resistance: sewage treatment plants (Paper IV) and a lake exposed to waste from pharmaceutical production (Paper V). There was no clear evidence for selection of antibiotic resistance genes in sewage treatment plants, however other factors such as oxygen availability seem to have much stronger effects on these microbial communities, which may mask small selective effects of antibiotics and other co-selective agents. In contrast, in the lake subjected to industrial pharmaceutical pollution, resistance genes and mobile genetic elements were both diverse and abundant. Finally, Paper VI shows that travel contributes to the spread of resistance genes against several different classes of antibiotics between countries with higher resistance rates and Sweden. In Papers IV–VI, the genetic contexts of resistance genes were assessed through metagenomic assembly, showing how different resistance genes are linked to each other in different environments. Through these means, the thesis contributes knowledge about risk settings for development and transmission of antibiotic resistance genes, which can be used to guide risk assessment and management schemes to delay or reduce clinical resistance development.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Biologiska vetenskaper ->
Biologiska vetenskaper ->
Bioinformatik och systembiologi
Medicinska grundvetenskaper ->
Mikrobiologi inom det medicinska området
antibiotic resistance, metagenomics, bioinformatics, next generation sequencing, resistance selection
Postens nummer:
Posten skapad:
2016-08-24 19:30

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007