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Preparation of Amplicon Libraries for Metabarcoding of Marine Eukaryotes Using Illumina MiSeq: The Adapter Ligation Method

Författare och institution:
Matthieu Leray (-); Quiterie Haenel (-); Sarah Bourlat (Institutionen för marina vetenskaper)
Publicerad i:
Marine Genomics: Methods and Protocols, s. 209-218
ISBN:
978-1-4939-3772-1
Publikationstyp:
Kapitel
Förlag:
Springer
Förlagsort:
New York
Publiceringsår:
2016
Språk:
engelska
Sammanfattning (abstract):
Amplicon-based studies of marine microscopic eukaryotes, also referred to as metabarcoding studies, can be performed to analyze patterns of biodiversity or predator–prey interactions targeting the mitochondrial cytochrome oxidase 1 (CO1) or the small ribosomal subunit (SSU) markers. Because high-throughput sequencing (HTS) Illumina platforms provide millions of reads per run, hundreds of samples may be sequenced simultaneously. This protocol details the preparation of multiplexed amplicon libraries for Illumina MiSeq sequencing. We describe a strategy for sample multiplexing using a combination of tailed PCR primers and ligation of indexed adapters.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
NATURVETENSKAP ->
Biologiska vetenskaper
NATURVETENSKAP ->
Biologiska vetenskaper ->
Biokemi och molekylärbiologi
NATURVETENSKAP ->
Biologiska vetenskaper ->
Genetik
Nyckelord:
Metabarcoding Eukaryotes Illumina MiSeq Multiplexing Tags TruSeq
Postens nummer:
240563
Posten skapad:
2016-08-22 11:03

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