transparent gif

 

Ej inloggad.

Göteborgs universitets publikationer

Elucidating selection processes for antibiotic resistance in sewage treatment plants using metagenomics

Författare och institution:
Johan Bengtsson-Palme (Institutionen för biomedicin, avdelningen för infektionssjukdomar); Rickard Hammarén (Institutionen för biomedicin, avdelningen för infektionssjukdomar); Chandan Pal (Institutionen för biomedicin, avdelningen för infektionssjukdomar); Marcus Östman (-); Berndt Björlenius (-); Carl-Fredrik Flach (Institutionen för biomedicin, avdelningen för infektionssjukdomar); Erik Kristiansson (Institutionen för matematiska vetenskaper, matematisk statistik, Chalmers/GU); Jerker Fick (-); Mats Tysklind (-); D. G. Joakim Larsson (Institutionen för biomedicin, avdelningen för infektionssjukdomar)
Publicerad i:
Science of the Total Environment, epub ahead of print
ISSN:
0048-9697
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Sewage treatment plants (STPs) have repeatedly been suggested as “hotspots” for the emergence and dissemination of antibiotic-resistant bacteria. A critical question still unanswered is if selection pressures within STPs, caused by residual antibiotics or other co-selective agents, are sufficient to specifically promote resistance. To address this, we employed shotgun metagenomic sequencing of samples from different steps of the treatment process in three Swedish STPs. In parallel, concentrations of selected antibiotics, biocides and metals were analyzed. We found that concentrations of tetracycline and ciprofloxacin in the influent were above predicted concentrations for resistance selection, however, there was no consistent enrichment of resistance genes to any particular class of antibiotics in the STPs, neither for biocide and metal resistance genes. The most substantial change of the bacterial communities compared to human feces occurred already in the sewage pipes, manifested by a strong shift from obligate to facultative anaerobes. Through the treatment process, resistance genes against antibiotics, biocides and metals were not reduced to the same extent as fecal bacteria. The OXA-48 gene was consistently enriched in surplus and digested sludge. We find this worrying as OXA-48, still rare in Swedish clinical isolates, provides resistance to carbapenems, one of our most critically important classes of antibiotics. Taken together, metagenomics analyses did not provide clear support for specific antibiotic resistance selection. However, stronger selective forces affecting gross taxonomic composition, and with that resistance gene abundances, limit interpretability. Comprehensive analyses of resistant/non-resistant strains within relevant species are therefore warranted.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
NATURVETENSKAP ->
Biologiska vetenskaper ->
Mikrobiologi
TEKNIK OCH TEKNOLOGIER ->
Miljöbioteknik ->
Vattenbehandling
MEDICIN OCH HÄLSOVETENSKAP ->
Klinisk medicin ->
Infektionsmedicin
Nyckelord:
Antibiotic resistance genes, Co-selection, Fecal bacteria, Microbial ecology, Risk assessment, Wastewater treatment
Postens nummer:
240554
Posten skapad:
2016-08-22 09:45
Posten ändrad:
2016-08-23 08:56

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007