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Göteborgs universitets publikationer

Insulin released from titanium discs with insulin coatings- kinetics and biological activity

Författare och institution:
Behnosh Öhrnell Malekzadeh (Institutionen för odontologi); Maria Ransjö (Institutionen för odontologi, sektion 3); Pentti Tengvall (Institutionen för kliniska vetenskaper, sektionen för anestesi, biomaterial och ortopedi, Avdelningen för biomaterialvetenskap); Z Mladenovic (Institutionen för odontologi, sektion 3); Anna Westerlund (Institutionen för odontologi, sektion 3)
Publicerad i:
Journal of Biomedical Materials Research. Part B - Applied biomaterials, Epub ahead of print
ISSN:
1552-4973
E-ISSN:
1552-4981
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Local administration of insulin from a titanium surface has been demonstrated to increase bone formation in non-diabetic rats. The authors hypothesised that insulin was released from the titanium surface and with preserved biological activity after the release. Thus, in the present in vitro study, human recombinant insulin was immobilized onto titanium discs, and the insulin release kinetics was evaluated using Electro-chemiluminescence immunoassay. Neutral Red uptake assay and a mineralization assay were used to evaluate the biological effects of insulin on human osteoblast-like MG-63 cells. The results confirmed that insulin was released from titanium surfaces during a six-week period. Furthermore, etching the disc prior to insulin coating, thickening of the insulin coating and incubation of the discs in serum-enriched cell culture medium increased the release. Another interesting finding was that longer storage time decreased the release of insulin. The released insulin had retained its biological activity, as demonstrated by the significant increase in cell number and a stimulated mineralization process, upon exposure to released insulin.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Hälsovetenskaper
Nyckelord:
Insulin, biomaterial, surface modification, osteoblast, titanium
Postens nummer:
240535
Posten skapad:
2016-08-20 11:06
Posten ändrad:
2016-08-22 11:07

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