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Heat-induced fragmentation and adapter-assisted whole genome amplification using GenomePlex® single-cell whole genome amplification kit (WGA4)

Författare och institution:
A. El-Heliebi (-); S. Chen (-); Thomas Kroneis (Sahlgrenska Cancer Center)
Publicerad i:
Whole Genome Amplification: Methods and Protocols, s. 101-109
ISBN:
978-149392990-0
Publikationstyp:
Kapitel
Förlag:
Springer
Förlagsort:
New York
Publiceringsår:
2015
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Whole genome amplification (WGA) is a widely used technique allowing multiplying picogram amounts of target DNA by several orders of magnitude. The technique described here is based on heat-induced random fragmentation yielding DNA strands mainly ranging from 0.1 to 1 kb in length. The fragmented DNA is then subjected to library generation by annealing of adaptor sequences to both ends of the DNA fragments. Using primers hybridizing to the adapter sequences, the DNA is amplified by thermal cycling. This amplification typically yields > 2 mg DNA from a single cell, is suited for amplifying DNA isolated from (partly) degraded samples [e.g. formalin-fixed paraffin-embedded (FFPE) material] and works well when used for array-comparative genome hybridization (array-CGH). © Springer Science+Business Media New York 2015. All rights reserved.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Medicinska grundvetenskaper ->
Medicinsk genetik
Nyckelord:
FFPE samples, GenomePlex, Single-cell analysis, Single-cell whole genome amplification, Whole genome amplification
Postens nummer:
240416
Posten skapad:
2016-08-18 15:22
Posten ändrad:
2016-08-18 15:24

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