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Göteborgs universitets publikationer

Cell saver processing mitigates the negative effects of wound blood on platelet function

Författare och institution:
J. Gabel (-); Carl Johan Malm (Institutionen för medicin, avdelningen för molekylär och klinisk medicin); V. Radulovic (-); Caroline Andersson Shams Hakimi (Institutionen för medicin, avdelningen för molekylär och klinisk medicin); M. Westerberg (-); Anders Jeppsson (Institutionen för medicin, avdelningen för molekylär och klinisk medicin)
Publicerad i:
Acta Anaesthesiologica Scandinavica, 60 ( 7 ) s. 901-909
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
BackgroundWound blood is highly activated and has poor haemostatic properties. Recent data suggest that retransfusion of unwashed wound blood may impair haemostasis. We hypothesized that cell saver processing of wound blood before retransfusion reduces the negative effects. MethodsWound blood was collected from 16 cardiac surgery patients during cardiopulmonary bypass. One portion of the wound blood was processed in a cell saver and one portion left unprocessed. Increasing amounts of unprocessed blood (10% and 20% of the systemic blood volume) or corresponding volumes of processed blood were added ex vivo to whole blood samples from the same patient. Clot formation was assessed by modified thromboelastometry (ROTEM (R)) and platelet function with impedance aggregometry (Multiplate((R))). ResultsAddition of unprocessed wound blood significantly impaired clot formation and platelet aggregability. Cell saver processing before addition did not influence clot formation but abolished completely the negative effects of wound blood on platelet aggregability tested with all agonists. Median adenosine diphosphate-induced platelet aggregation was 51 (25th and 75th percentiles 42-69) when 20% processed cardiotomy suction blood was added vs. 34 (24-52) U when 20% unprocessed blood was added, P < 0.001. The corresponding figures for arachidonic acid-, thrombin receptor activating peptide- and collagen-induced aggregation was 21 (17-51) vs. 13 (10-25) U, 112 (87-128) vs. 78 (65-103) U and 58 (50-73) vs. 33 (28-44) U, respectively, all P < 0.001). ConclusionThe results suggest that cell saver processing before retransfusion mitigates the negative effects of wound blood on platelet function despite that cell saver processing reduces platelet count.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin ->
Anestesi och intensivvård ->
cardiotomy suction blood, cardiopulmonary bypass, cardiac-surgery, whole-blood, ex-vivo, autotransfusion, activation, thromboelastometry, coagulation, trial, Anesthesiology
Postens nummer:
Posten skapad:
2016-08-11 13:50
Posten ändrad:
2016-08-11 13:51

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