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Göteborgs universitets publikationer

ELISPOT assay for measurement of antigen-specific and polyclonal antibody responses

Författare och institution:
Nils Y Lycke (Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi); R. Coico (-)
Publicerad i:
Current Protocols in Immunology, 2015 s. 7.14.1-7.14.10
ISSN:
1934-3671
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2015
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
The enzyme-linked immunospot (ELISPOT) assay for detection of antigenspecific and polyclonal antibody responses by single antibody-secreting cells has become the method of choice due to its cell-based quantitative value. Antigen stability and specificity and the diversity of antigens that can be used in the assay have contributed to the translational application of ELISPOT as demonstrated by many FDA-approved clinical tests that employ this technique. In addition, the ELISPOT assay can be used to detect two antigenically different secreted antibodies simultaneously by two-color analysis and offers the unique possibility of quantifying the number of antibody molecules secreted per cell. © 2015 by John Wiley & Sons, Inc.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Medicinska grundvetenskaper ->
Immunologi inom det medicinska området
Nyckelord:
Antibody-secreting cells, ELISA, ELISPOT
Postens nummer:
240039
Posten skapad:
2016-08-09 13:47
Posten ändrad:
2016-08-09 13:48

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