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Göteborgs universitets publikationer

Microdialysis and proteomics of subcutaneous interstitial fluid reveals increased galectin-1 in type 2 diabetes patients

Författare och institution:
Emanuel Fryk (Institutionen för medicin, avdelningen för molekylär och klinisk medicin & Wallenberglaboratoriet); Jeanna Perman Sundelin (Institutionen för medicin, avdelningen för molekylär och klinisk medicin & Wallenberglaboratoriet); Lena Strindberg (Institutionen för medicin, avdelningen för molekylär och klinisk medicin & Wallenberglaboratoriet); Maria J Pereira (Institutionen för medicin, avdelningen för molekylär och klinisk medicin); M. Federici (-); N. Marx (-); Helena Filipsson Nyström (Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition); M. Schmelz (-); Per-Arne Svensson (Institutionen för medicin, avdelningen för molekylär och klinisk medicin & Wallenberglaboratoriet); Jan W Eriksson (-); Jan Borén (Institutionen för medicin, avdelningen för molekylär och klinisk medicin & Wallenberglaboratoriet); Per-Anders Jansson (Institutionen för medicin, avdelningen för molekylär och klinisk medicin & Wallenberglaboratoriet)
Publicerad i:
Metabolism-Clinical and Experimental, 65 ( 7 ) s. 998-1006
ISSN:
0026-0495
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Objective. To identify a potential therapeutic target for type 2 diabetes by comparing the subcutaneous interstitial fluid from type 2 diabetes patients and healthy men. Methods. Proteomics was performed on the interstitial fluid of subcutaneous adipose tissue obtained by microdialysis from 7 type 2 diabetes patients and 8 healthy participants. 851 proteins were detected, of which 36 (including galectin-1) showed significantly altered expression in type 2 diabetes. We also measured galectin-1 expression in: (1) adipocytes isolated from adipose tissue biopsies from these participants; (2) subcutaneous adipose tissue of 24 obese participants before, during and after 16 weeks on a very low calorie diet (VLCD); and (3) adipocytes isolated from 6 healthy young participants after 4 weeks on a diet and lifestyle intervention to promote weight gain. We also determined the effect of galectin-1 on glucose uptake in human adipose tissue. Results. Galectin-1 protein levels were elevated in subcutaneous dialysates from type 2 diabetes compared with healthy controls (p < 0.05). In agreement, galectin-1 mRNA expression was increased in adipocytes from the type 2 diabetes patients (p < 0.05). Furthermore, galectin-1 mRNA expression was decreased in adipose tissue after VLCD (p < 0.05) and increased by overfeeding (p < 0.05). Co-incubation of isolated human adipocytes with galectin-1 reduced glucose uptake (p < 0.05) but this was independent of the insulin signal. Conclusion. Proteomics of the interstitial fluid in subcutaneous adipose tissue in vivo identified a novel adipokine, galectin-1, with a potential role in the pathophysiology of type 2 diabetes. (C) 2016 Elsevier Inc. All rights reserved.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Klinisk medicin
Nyckelord:
Microdialysis, Proteomics, Galectin-1, Obesity, Adipose tissue, induced obese rats, body-weight gain, adipose-tissue, insulin-resistance, gene-expression, targeted inhibition, skeletal-muscle, identification, proteins, glucose, keigue pm, 1991, lancet, v337, p382
Postens nummer:
240025
Posten skapad:
2016-08-09 11:34

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