transparent gif

 

Ej inloggad.

Göteborgs universitets publikationer

Optogenetic Control of Pancreatic Islets

Författare och institution:
Thomas Reinbothe (Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi); I. G. Mollet (-)
Publicerad i:
Methods Mol Biol, 1408 s. 107-23
ISSN:
1940-6029 (Electronic) 1064-3745 (Linking)
Publikationstyp:
Kapitel, refereegranskat
Förlag:
Springer
Förlagsort:
Heidelberg
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
In light of the emerging diabetes epidemic, new experimental approaches in islet research are needed to elucidate the mechanisms behind pancreatic islet dysfunction and to facilitate the development of more effective therapies. Optogenetics has created numerous new experimental tools enabling us to gain insights into processes little was known about before. The spatial and temporal precision that it can achieve is also attractive for studying the cells of the pancreatic islet and we set out to explore the possibilities of this technology for our purposes. We here describe how to use the islets of an "optogenetic beta-cell" mouse line in islet batch incubations and Ca(2+) imaging experiments. This protocol enables light-induced insulin release and provides an all-optical solution to control and measure intracellular Ca(2+) levels in pancreatic beta-cells. The technique is easy to set up and provides a useful tool for controlling the activity of distinct islet cell populations.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Medicinska grundvetenskaper ->
Cell- och molekylärbiologi ->
Cellbiologi ->
Medicinsk cellbiologi
Nyckelord:
All-optical, Beta-cell, Calcium, Channelrhodopsin-2, Diabetes, Insulin, Islets, Optogenetics, Pancreas
Postens nummer:
240010
Posten skapad:
2016-08-09 11:18

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007