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Göteborgs universitets publikationer

BBX21, an Arabidopsis B-box protein, directly activates HY5 and is targeted by COP1 for 26S proteasome-mediated degradation

Författare och institution:
D. Q. Xu (-); Y. Jiang (-); J. G. Li (-); F. Lin (-); Magnus Holm (Institutionen för biologi och miljövetenskap); X. W. Deng (-)
Publicerad i:
Proceedings of the National Academy of Sciences of the United States of America, 113 ( 27 ) s. 7655-7660
ISSN:
0027-8424
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
BBX)-containing protein, has been previously identified as a positive regulator of light signaling; however, the precise role of BBX21 in regulating seedling photomorphogenesis remains largely unclear. In this study, we report that CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) interacts with BBX21 in vivo and is able to ubiquitinate BBX21 in vitro. Thus, BBX21 is targeted for 26S proteasome-mediated degradation in dark-grown Arabidopsis seedlings in a COP1-dependent manner. Moreover, we show that BBX21 binds to the T/G-box in the ELONGATED HYPOCOTYL 5 (HY5) promoter and directly activates HY5 expression in the light. Transgenic seedlings overexpressing BBX21 exhibit dramatically shortened hypocotyls in the light, and this phenotype is dependent on a functional HY5. Taken together, our data suggest a molecular base underlying BBX21-mediated seedling photomorphogenesis, indicating that BBX21 is a pivotal component involved in the COP1-HY5 regulatory hub.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
NATURVETENSKAP ->
Biologiska vetenskaper
Nyckelord:
Arabidopsis, photomorphogenesis, BBX21, COP1, HY5, seedling development, gene-expression, cop1-mediated ubiquitination, elongated hypocotyl5, physically interact, light, photomorphogenesis, transcription, regulator, abundance
Postens nummer:
239628
Posten skapad:
2016-07-28 14:19

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