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Foxf2 is required for secondary palate development and Tgf beta signaling in palatal shelf mesenchyme

Författare och institution:
Ali Moussavi Nik (Institutionen för kemi och molekylärbiologi); JA Johansson (Institutionen för kemi och molekylärbiologi); Mozhgan Ghiami (Institutionen för kemi och molekylärbiologi); Azadeh Reyahi (Institutionen för kemi och molekylärbiologi); P. Carlsson (Institutionen för kemi och molekylärbiologi)
Publicerad i:
Developmental Biology, 415 ( 1 ) s. 14-23
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The secondary palate separates the oral from the nasal cavity and its closure during embryonic development is sensitive to genetic perturbations. Mice with deleted Foxf2, encoding a forkhead transcription factor, are born with cleft palate, and an abnormal tongue morphology has been proposed as the underlying cause. Here, we show that Foxf2(-/-) maxillary explants cultured in vitro, in the absence of tongue and mandible, failed to close the secondary palate. Proliferation and collagen content were decreased in Foxf2(-/-) palatal shelf mesenchyme. Phosphorylation of Smad2/3 was reduced in mutant palatal shelf, diagnostic of attenuated canonical Tgf beta signaling, whereas phosphorylation of p38 was increased. The amount of Tgf beta 2 protein was diminished, whereas the Tgfb2 mRNA level was unaltered. Expression of several genes encoding extracellular proteins important for Tgf beta signaling were reduced in Foxf2(-/-) palatal shelves: a fibronectin splice-isoform essential for formation of extracellular Tgf beta latency complexes; Tgfbr3 - or betaglycan - which acts as a co-receptor and an extracellular reservoir of Tgf beta; and integrins alpha V and beta 1, which are both Tgf beta targets and required for activation of latent Tgf beta. Decreased proliferation and reduced extracellular matrix content are consistent with diminished Tgf beta signaling. We therefore propose that gene expression changes in palatal shelf mesenchyme that lead to reduced Tgf beta signaling contribute to cleft palate in Foxf2(-/-) mice.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Biologiska vetenskaper
transcription factor foxf2, causes cleft-palate, forkhead gene, neural, crest, epithelial proliferation, differential expression, tgf-beta-1, activation, mouse embryos, mice lacking, mutant mice, Developmental Biology, oetzel g, 1995, nature genetics, v11, p409, reman dm, 1991, archives of oral biology, v36, p457
Postens nummer:
Posten skapad:
2016-07-26 16:13

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