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Göteborgs universitets publikationer

Expression of inflammation/pain-related genes in the dorsal root ganglion following disc puncture in rats

Författare och institution:
Yuki Fujioka (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Anders Ståhlberg (Institutionen för biomedicin, avdelningen för patologi); M. Ochi (-); Kjell Olmarker (Institutionen för biomedicin)
Publicerad i:
Journal of Orthopaedic Surgery, 24 ( 1 ) s. 106-112
ISSN:
1022-5536
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Sammanfattning (abstract):
Purpose. To determine the expression of inflammation- and pain-related genes at days 1 and 3. in the dorsal root ganglion (DRG) of rats with or without disc puncture, using real-time quantitative polymerase chain reaction (RT-qPCR) with the TaqMan low-density array (TLDA). Methods. 53 female Sprague-Dawley rats were used. The left facet joint between L4 and L5 was removed, and the DRG and intervertebral disc between the vertebrae were exposed. The L4-5 intervertebral disc was punctured using a 0.4-mm diameter injection needle (disc puncture group) or left unpunctured (sham group). After one or 3 days, the 53 DRGs were harvested, frozen, and assessed for expression of inflammation-related genes. Total RNA was isolated from the DRGs. Expression of 119 genes related to inflammation and pain in the DRG after disc puncture were analysed using RT-qPCR with the TLDA. Results. Of the 95 inflammation-related genes, 78 genes were reliably detected. Two genes were significantly up-regulated: cysteinyl leukotriene receptor 1 (CYSLTR1) at day 3 and interleukin 2 receptor gamma (IL2RG) at day 1, and one gene was significantly down-regulated: phospholipase C beta 3 (PLCB3) at day 1. Of the 24 pa in-related genes, 18 genes were reliably detected. Two genes were significantly up-regulated: nitric oxide synthase 1 (NOS1) at days 1 and 3 and 5-HT2A receptor (HTR2A) at day 1. Conclusion. Disc puncture may elicit changes in the expression of a variety of genes. Gene expression profiling is a useful tool for detecting new potential pharmaceutical targets for spinal pain syndromes.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Klinisk medicin ->
Ortopedi
Nyckelord:
ganglia, spinal, gene expression profiling, low back pain, nucleus-pulposus, nerve root, receptor, pain, cells, Orthopedics, Surgery
Postens nummer:
238976
Posten skapad:
2016-07-05 13:41

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