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Göteborgs universitets publikationer

Lactobacilli Reduce Helicobacter pylori Attachment to Host Gastric Epithelial Cells by Inhibiting Adhesion Gene Expression

Författare och institution:
N. de Klerk (-); L. Maudsdotter (-); H. Gebreegziabher (-); S. D. Saroj (-); B. Eriksson (-); O. S. Eriksson (-); S. Roos (-); Sara K. Lindén (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); H. Sjolinder (-); A. B. Jonsson (-)
Publicerad i:
Infection and Immunity, 84 ( 5 ) s. 1526-1535
ISSN:
0019-9567
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
The human gastrointestinal tract, including the harsh environment of the stomach, harbors a large variety of bacteria, of which Lactobacillus species are prominent members. The molecular mechanisms by which species of lactobacilli interfere with pathogen colonization are not fully characterized. In this study, we aimed to study the effect of lactobacillus strains upon the initial attachment of Helicobacter pylori to host cells. Here we report a novel mechanism by which lactobacilli inhibit adherence of the gastric pathogen H. pylori. In a screen with Lactobacillus isolates, we found that only a few could reduce adherence of H. pylori to gastric epithelial cells. Decreased attachment was not due to competition for space or to lactobacillus-mediated killing of the pathogen. Instead, we show that lactobacilli act on H. pylori directly by an effector molecule that is released into the medium. This effector molecule acts on H. pylori by inhibiting expression of the adhesin-encoding gene sabA. Finally, we verified that inhibitory lactobacilli reduced H. pylori colonization in an in vivo model. In conclusion, certain Lactobacillus strains affect pathogen adherence by inhibiting sabA expression and thereby reducing H. pylori binding capacity.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Klinisk medicin
Nyckelord:
signal-transduction system, human stomach, in-vitro, binding, saba, disease, strain, mucins, model, colonization, Immunology, Infectious Diseases
Postens nummer:
238948
Posten skapad:
2016-07-05 11:59

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