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The Bacterial (Vibrio alginolyticus) Production of Tetrodotoxin in the Ribbon Worm Lineus longissimus-Just a False Positive?

Författare och institution:
M. Strand (-); M. Hedstrom (-); Henrik Seth (Institutionen för biologi och miljövetenskap); E. G. McEvoy (-); E. Jacobsson (-); U. Goransson (-); H. S. Andersson (-); Per Sundberg (Institutionen för biologi och miljövetenskap)
Publicerad i:
Marine Drugs, 14 ( 4 )
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel-dependent nerve conductance in maleWistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and death likely emanate from a compound <5 kDa, and via a different mechanism of action than that of TTX. The similarity in mass between TTX and the Vibrio-produced low-molecular-weight, non-toxic compound invokes that thorough analysis is required when assessing TTX production. Based on our findings, we suggest that re-examination of some published claims of TTX production may be warranted.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Geovetenskap och miljövetenskap ->
Oceanografi, hydrologi, vattenresurser
LC-MS, mucus, axonal conductance, Vibrio, nemertean, tetrodotoxin, gated sodium-channels, hiroshima prefecture, cephalothrix-simula, puffer, fishes, japan, bay, platyhelminthes, polycladida, substances, toxicity, Pharmacology & Pharmacy, yazawa k, 1988, toxicon, v26, p867, llens me, 1992, proceedings of the national academy of sciences of the united states of
Postens nummer:
Posten skapad:
2016-06-09 14:40

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