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Göteborgs universitets publikationer

The Evolutionarily Conserved Protein PHOTOSYNTHESIS AFFECTED MUTANT71 Is Required for Efficient Manganese Uptake at the Thylakoid Membrane in Arabidopsis

Författare och institution:
A. Schneider (-); I. Steinberger (-); Andrei Herdean (Institutionen för biologi och miljövetenskap); C. Gandini (-); M. Eisenhut (-); S. Kurz (-); A. Morper (-); N. Hoecker (-); T. Ruhle (-); M. Labs (-); U. I. Flugge (-); S. Geimer (-); S. B. Schmidt (-); S. Husted (-); A. P. M. Weber (-); Cornelia Spetea (Institutionen för biologi och miljövetenskap); D. Leister (-)
Publicerad i:
Plant Cell, 28 ( 4 ) s. 892-910
ISSN:
1040-4651
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
In plants, algae, and cyanobacteria, photosystem II (PSII) catalyzes the light-driven oxidation of water. The oxygen-evolving complex of PSII is a Mn4CaO5 cluster embedded in a well-defined protein environment in the thylakoid membrane. However, transport of manganese and calcium into the thylakoid lumen remains poorly understood. Here, we show that Arabidopsis thaliana PHOTOSYNTHESIS AFFECTED MUTANT71 (PAM71) is an integral thylakoid membrane protein involved in Mn2+ and Ca2+ homeostasis in chloroplasts. This protein is required for normal operation of the oxygen-evolving complex (as evidenced by oxygen evolution rates) and for manganese incorporation. Manganese binding to PSII was severely reduced in pam71 thylakoids, particularly in PSII supercomplexes. In cation partitioning assays with intact chloroplasts, Mn2+ and Ca2+ ions were differently sequestered in pam71, with Ca2+ enriched in pam71 thylakoids relative to the wild type. The changes in Ca2+ homeostasis were accompanied by an increased contribution of the transmembrane electrical potential to the proton motive force across the thylakoid membrane. PSII activity in pam71 plants and the corresponding Chlamydomonas reinhardtii mutant cgld1 was restored by supplementation with Mn2+, but not Ca2+. Furthermore, PAM71 suppressed the Mn2+-sensitive phenotype of the yeast mutant Delta pmr1. Therefore, PAM71 presumably functions in Mn2+ uptake into thylakoids to ensure optimal PSII performance.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
NATURVETENSKAP ->
Biologiska vetenskaper
Nyckelord:
synechocystis sp pcc-6803, evolving photosystem-ii, kda extrinsic, protein, chlorophyll-fluorescence, oxygenic photosynthesis, plant, transformation, secretory pathway, redox regulation, calcium-binding, gamma-subunit
Postens nummer:
237142
Posten skapad:
2016-05-31 15:44

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