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Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding

Författare och institution:
I. Bjorkgren (-); L. Alvarez (-); N. Blank (-); M. Balbach (-); H. Turunen (-); T. D. Laajala (-); J. Toivanen (-); A. Krutskikh (-); N. Wahlberg (-); I. Huhtaniemi (-); Matti Poutanen (Institutionen för medicin); D. Wachten (-); P. Sipila (-)
Publicerad i:
Molecular and Cellular Endocrinology, 427 ( C ) s. 143-154
ISSN:
0303-7207
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
During epididymal maturation, sperm acquire the ability to swim progressively by interacting with proteins secreted by the epididymal epithelium. Beta-defensin proteins, expressed in the epididymis, continue to regulate sperm motility during capacitation and hyperactivation in the female reproductive tract. We characterized the mouse beta-defensin 41 (DEFB41), by generating a mouse model with iCre recombinase inserted into the first exon of the gene. The homozygous Defb41(iCre/iCre) knock-in mice lacked Defb41 expression and displayed iCre recombinase activity in the principal cells of the proximal epididymis. Heterozygous Defb41(iCre/+) mice can be used to generate epididymis specific conditional knock-out mouse models. Homozygous Defb41(iCre/iCre) sperm displayed a defect in sperm motility with the flagella primarily bending in the pro-hook conformation while capacitated wild-type sperm more often displayed the anti-hook conformation. This led to a reduced straight line motility of Defb41(iCre/liCre) sperm and weaker binding to the oocyte. Thus, DEFB41 is required for proper sperm maturation.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Klinisk medicin
Nyckelord:
Beta-defensin, Epididymis, Sperm maturation, Flagellar motility pattern, Sperm-oocyte binding, iCre knock-in, tyrosine phosphorylation, hyperactivated motility, rat spermatozoa, male-fertility, movement characteristics, reproductive-tract, gene, clusters, protein, capacitation, mice, Cell Biology
Postens nummer:
237065
Posten skapad:
2016-05-30 11:18

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